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Invitrogen
GITRL Polyclonal Antibody
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FIGURE: 1 / 1
GITRL Antibody (PA5-96552) in WB
Western blot analysis of GITRL in rat skeletal muscle. Samples were incubated with GITRL Polyclonal antibody (Product # PA5-96552) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-96552
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 52-177 of human GITR Ligand/TNFSF18 (NP_0050832)
Conjugate
Form
Liquid
Concentration
2.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: SW620, SKOV3, Mouse skeletal muscle, Rat skeletal muscle; Cellular Location: Cell membrane, Single-pass type II membrane protein
Immunogen sequence: CSIVMLLFLC SFSWLIFIFL QLETAKEPCM AKFGPLPSKW QMASSEPPCV NKVSDWKLEI LQNGLYLIYG QVAPNANYND VAPFEVRLYK NKDMIQTLTN KSKIQNVGGT YELHVGDTID LIFNSE
Target Information
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kappa-B and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-kappa-B, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-kappa-B activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation-inducible TNF-related ligand; AITR ligand; AITRL; GITR ligand; GITRL; GITRLigand; Glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; glucocorticoid-induced-tumor necrosis factor receptor ligand; hGITRL; RP1-15D23.1; TNF superfamily member 18; tumor necrosis factor (ligand) superfamily member 18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand 2A; Tumor necrosis factor ligand superfamily member 18
Gene Aliases: AITRL; GITRL; hGITRL; TL6; TNFSF18; TNLG2A; UNQ149/PRO175
UniProt ID: (Human) Q9UNG2, (Mouse) Q7TS55
Entrez Gene ID: (Human) 8995, (Mouse) 240873, (Rat) 364031
T cell proliferation involved in immune response
immune system process
positive regulation of leukocyte migration
immune response
cell-cell signaling
positive regulation of macrophage chemotaxis
interleukin-10 production
interleukin-12 production
regulation of interferon-gamma production
regulation of interleukin-6 production
regulation of tumor necrosis factor production
tumor necrosis factor-mediated signaling pathway
activated CD4-positive, alpha-beta T cell proliferation
regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of cell adhesion
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
regulation of monocyte chemotactic protein-1 production
positive regulation of monocyte chemotaxis
positive regulation of protein homodimerization activity
negative regulation of T-helper 17 cell lineage commitment
regulation of CD40 signaling pathway
regulation of dendritic cell chemotaxis
adaptive immune response
signal transduction
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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