Invitrogen

GJA10 Polyclonal Antibody

5 Published Figures

View all (4) GJA10 antibodies

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GJA10 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunofluorescent staining of frozen mouse retina using Zymed Rb anti-Connexin 57 (Mid) (Product # 40-5000). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

GJA10 Antibody in Immunohistochemistry (IHC) — Immunofluorescence analysis of Connexin57 (Cx57) was performed on sections of adult mouse retina. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit polyclonal anti-Cx57 (Product # 40-5000) at an antibody concentration of 1-2 µg/mL diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then washed in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss 710 confocal microscope at x40 objective magnification, and show immunofluorescence labelling of Cx57 localized at gap junctions between processes of horizontal cells in the outer plexiform layer of adult mouse retina. Data courtesy of Dr. James Nagy's lab.

GJA10 Antibody in Immunocytochemistry (ICC/IF) — Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunocytochemistry

GJA10 Antibody in Immunohistochemistry (IHC) — Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunohistochemistry

GJA10 Antibody in Western Blot (WB) — Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Western Blot

GJA10 Antibody in Western Blot (WB) — Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Western Blot

GJA10 Antibody in Immunomicroscopy (IM) — Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Product Details

40-5000

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the mouse, rat, Chinese hamster and golden hamster connexin 57 (Cx57) proteins

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533469

Product Specific Information

40-5000 was used in immunofluorescence analysis of Connexin57 (Cx57) on sections of adult mouse retina.

Target Information

Connexins, such as GJA10, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

The Journal of comparative neurology

Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC

Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Sun Jul 01 00:00:00 EDT 2012

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

The Journal of comparative neurology

Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC

Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Sun Jul 01 00:00:00 EDT 2012

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

The Journal of comparative neurology

Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC

Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Sun Jul 01 00:00:00 EDT 2012

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

The Journal of comparative neurology

Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC

Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Sun Jul 01 00:00:00 EDT 2012

Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.

The Journal of comparative neurology

Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC

Published figure using GJA10 polyclonal antibody (Product # 40-5000) in Immunomicroscopy

Sun Jul 01 00:00:00 EDT 2012

Connexin-mediated communication controls cell proliferation and is essential in retinal histogenesis.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Kihara AH,Santos TO,Osuna-Melo EJ,Paschon V,Vidal KS,Akamine PS,Castro LM,Resende RR,Hamassaki DE,Britto LR

Mon Feb 01 00:00:00 EST 2010

Lack of photoreceptor signaling alters the expression of specific synaptic proteins in the retina.

Neuroscience

Kihara AH,Santos TO,Paschon V,Matos RJ,Britto LR

Tue Feb 19 00:00:00 EST 2008

Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina.

Neuroscience

Ciolofan C,Lynn BD,Wellershaus K,Willecke K,Nagy JI

40-5000 was used in immunohistochemistry and western blot to investigate connexin57 expression in the mouse retina

Fri Aug 24 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: connexin 62; Connexin-57; Connexin-62; Cx57; Cx62; Gap junction alpha-10 protein; gap junction membrane channel protein alpha 10; gap junction protein, alpha 10, 62kDa

Gene Aliases: Cx-57; cx57; Cx59; CX62; GJA10

UniProt ID: (Human) Q969M2, (Mouse) Q9WUS4

Entrez Gene ID: (Human) 84694, (Mouse) 14610

Molecular Function: gap junction

cell communication gamete generation synapse assembly detection of light stimulus involved in visual perception transmembrane transport response to light stimulus

Process(es)

gap junction channel activity