Proteintech

GJB3 Polyclonal Antibody

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GJB3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 12880-1-AP (GJB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GJB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

GJB3 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GJB3 Antibody in Immunoprecipitation (IP) — IP result of anti-GJB3 (IP:12880-1-AP, 4ug; Detection:12880-1-AP 1:500) with HeLa cells lysate 2600 ug.

Product Details

12880-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Chimpanzee, Human, Mouse, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GJB3 Fusion Protein Ag3839 (101-270 aa encoded by BC012918) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RERRHRQKH GDQCAKLYDN AGKKHGGLWW TYLFSLIFKL IIEFLFLYLL HTLWHGFNMP RLVQCANVAP CPNIVDCYIA RPTEKKIFTY FMVGASAVCI VLTICELCYL ICHRVLRGLH KDKPRGGCSP SSSASRASTC RCHHKLVEAG EVDPDPGNNK LQASAPNLTP I (101-270 aa encoded by BC012918)

Target Information

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparative analysis of molecular signatures suggests the use of gabapentin for the management of endometriosis-associated pain.

Journal of pain research

Bellessort B,Bachelot A,Grouthier V,De Lombares C,Narboux-Neme N,Garagnani P,Pirazzini C,Astigiano S,Mastracci L,Fontaine A,Alfama G,Duvernois-Berthet E,Levi G

Thu Mar 17 00:00:00 EDT 2022

Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.

Cell reports

Hosoya M,Fujioka M,Sone T,Okamoto S,Akamatsu W,Ukai H,Ueda HR,Ogawa K,Matsunaga T,Okano H

Tue Jan 03 00:00:00 EST 2017

Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.

Scientific reports

Hosoya M,Fujioka M,Ogawa K,Okano H

Fri Feb 26 00:00:00 EST 2016

Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.

Scientific reports

Hosoya M,Fujioka M,Ogawa K,Okano H

Fri Feb 26 00:00:00 EST 2016

Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.

Scientific reports

Hosoya M,Fujioka M,Ogawa K,Okano H

Fri Feb 26 00:00:00 EST 2016

FBXL12-Mediated Degradation of ALDH3 is Essential for Trophoblast Differentiation During Placental Development.

Stem cells (Dayton, Ohio)

Nishiyama M,Nita A,Yumimoto K,Nakayama KI

Sun Nov 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: connexin 31; Connexin-31; Cx31; Gap junction beta-3 protein; gap junction membrane channel protein beta 3; gap junction protein, beta 3, 31kDa

Gene Aliases: Cnx31; CX31; Cxn-31; D4Wsu144e; DFNA2; DFNA2B; EKV; Gjb-3; GJB3

UniProt ID: (Human) O75712, (Mouse) P28231, (Rat) P25305

Entrez Gene ID: (Human) 2707, (Mouse) 14620, (Rat) 29585

Molecular Function: gap junction