Proteintech

GLAST Polyclonal Antibody

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GLAST Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain tissue slide using 20785-1-AP ( GLAST antibody at dilution of 1:50 (under 40x lens).

GLAST Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GLAST Antibody in Immunoprecipitation (IP) — IP result of anti-GLAST (IP:20785-1-AP, 4ug; Detection:20785-1-AP 1:500) with mouse brain tissue lysate 3000ug.

Product Details

20785-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GLAST Fusion Protein Ag14177 (471-542 aa encoded by BC037310)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: VDWFLDRLR TTTNVLGDSL GAGIVEHLSR HELKNRDVEM GNSVIEENEM KKPYQLIAQD NETEKPIDSE TKM (471-542 aa encoded by BC037310)

Target Information

This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EAAT-1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST-1; Glial glutamate transporter; Glial high affinity glutamate transporter; GluT-1; glutamate transporter; glutamate/aspartate transporter; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3; solute carrier family 1, member 3

Gene Aliases: AI504299; B430115D02Rik; EA6; EAAT1; GLAST; GLAST-1; GLAST1; GLU-T; GluT-1; Gmt1; MGluT1; SLC1A3

UniProt ID: (Human) P43003, (Rat) P24942, (Mouse) P56564

Entrez Gene ID: (Human) 6507, (Rat) 29483, (Mouse) 20512

Molecular Function: primary active transporter