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Invitrogen
GLI2 Polyclonal Antibody
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FIGURE: 1 / 1
GLI2 Antibody (PA5-47500) in ChIP
ChIP assay of GLI2 in Jurkat human acute T cell leukemia cell line. Samples were immunoprecipitated with GLI2 polyclonal antibody (Product # PA5-47500) for 15 minutes in an ultrasonic bath using a dilution of 5 μg followed by a Biotinylated Anti-Sheep IgG secondary antibody. Cell line was treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid and DNA w... View More
Product Details
PA5-47500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human GLI-2 Ala2-Glu91
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; transcription factor; unnamed protein product; Zinc finger protein GLI2
Gene Aliases: CJS; GLI2; HPE9; PHS2; THP; THP1; THP2
UniProt ID: (Human) P10070
Entrez Gene ID: (Human) 2736
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
osteoblast differentiation
kidney development
osteoblast development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
smoothened signaling pathway
pattern specification process
axon guidance
ventral midline development
hindgut morphogenesis
heart development
anatomical structure morphogenesis
epidermal cell differentiation
proximal/distal pattern formation
regulation of gene expression
floor plate formation
spinal cord dorsal/ventral patterning
ventral spinal cord development
cerebellar cortex morphogenesis
smoothened signaling pathway involved in ventral spinal cord interneuron specification
spinal cord ventral commissure morphogenesis
pituitary gland development
lung development
mammary gland development
hindbrain development
hair follicle morphogenesis
positive regulation of T cell differentiation in thymus
tube development
odontogenesis of dentin-containing tooth
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
developmental growth
neuron development
branching morphogenesis of an epithelial tube
cellular response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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