Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Product Specifications | |
---|---|
Species Reactivity |
Avian, Dog, Chicken, Chimpanzee, Human, Xenopus |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Gli-3 protein. A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GLI-Kruppel family member 3; GLI-Kruppel family member GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome); GLI3 C-terminally truncated form; GLI3 form of 190 kDa; GLI3 form of 83 kDa; GLI3 full length protein; GLI3 full-length protein; GLI3-190; GLI3-83; GLI3FL; glioma-associated oncogene family zinc finger 3; neural specific DNA binding protein; Neural-specific DNA-binding protein xGLI3; oncogene GLI3; Transcriptional activator GLI3; Transcriptional repressor GLI3R; xGLI-3; Zinc finger protein GLI3
Gene Aliases: ACLS; GCPS; GLI3; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV; XELAEV_18031191mg; xgli3
UniProt ID: (Human) P10071, (Chicken) Q9IA31, (Chimpanzee) Q5IS56, (Xenopus) Q91660
Entrez Gene ID: (Dog) 483244, (Human) 2737, (Chicken) 420769, (Chimpanzee) 463369, (Xenopus) 397837
Molecular Function:
DNA-binding transcription factor
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support