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Invitrogen
GLRA1 Polyclonal Antibody
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FIGURE: 1 / 1
GLRA1 Antibody (PA1-4661) in WB
Western blot of GLRA1 in rat spinal cord lysate showing specific immunolabeling of a band at ~48 kDa corresponding to the alpha 1 and alpha 2-subunits of GLRA1 polyclonal antibody (Product # PA1-4661). Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.
Product Details
PA1-4661
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the N-terminal region of rat GLRA1 conjugated to KLH
Conjugate
Form
Lyophilized
Concentration
4 mg/mL
Purification
Affinity chromatography
Storage buffer
5mM Ammonium bicarbonate, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts, and does not recognize other Glycine Receptor subunits. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen.
Reconstitute with 100 µL PBS.
Target Information
GLRA1 is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in GLRA1 are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found for GLRA1. Glycine receptors (GlyR) that include GLRA1 are members of the cys-loop family of ligand-gated ion channels, responsible for mediating the inhibitory effects of glycine. GLRA1 are widely distributed throughout the CNS, particularly within the hippocampus, spinal cord and brain stem. Diseases associated with GLRA1 include Hyperekplexia, Hereditary 1 and Hyperekplexia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GLRA; Glra2; glycine receptor; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; glycine receptor, alpha 1 subunit; gra1
Gene Aliases: GLRA1; Glyr; GLYRA1; HKPX1; nmf11; oscillator; ot; spasmodic; spd; STHE
UniProt ID: (Human) P23415, (Mouse) Q64018, (Rat) P07727
Entrez Gene ID: (Human) 2741, (Mouse) 14654, (Rat) 25674
protein binding
zinc ion binding
glycine binding
extracellular-glycine-gated chloride channel activity
transmitter-gated ion channel activity
taurine binding
extracellular ligand-gated ion channel activity
chloride channel activity
extracellular-glycine-gated ion channel activity
ligand-gated ion channel
action potential
startle response
regulation of respiratory gaseous exchange by neurological system process
ion transport
chloride transport
muscle contraction
neuropeptide signaling pathway
acrosome reaction
visual perception
adult walking behavior
ion transmembrane transport
regulation of membrane potential
neuromuscular process controlling posture
protein homooligomerization
protein heterooligomerization
negative regulation of transmission of nerve impulse
synaptic transmission, glycinergic
righting reflex
inhibitory postsynaptic potential
cellular response to amino acid stimulus
cellular response to zinc ion
cellular response to ethanol
response to alcohol
chloride transmembrane transport
positive regulation of acrosome reaction
transport
anion transport
chemical synaptic transmission
regulation of respiratory gaseous exchange
neuromuscular process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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