Invitrogen

GLUT1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

16 References

View all (32) GLUT1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GLUT1 in formalin-fixed paraffin-embedded tissue section of human placenta. Samples were incubated in GLUT1 polyclonal antibody (Product # PA1-46152) using a dilution of 1:200. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.

GLUT1 Antibody in Western Blot (WB) — Western blot was performed using Anti-GLUT1 Polyclonal Antibody (Product # PA1-46152) and a ~55 kDa band corresponding to GLUT1 was observed across tissues tested . Membrane enriched extracts (30 µg lysate) of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GLUT1 Antibody in Immunohistochemistry (IHC) — Figure 8 Immunohistochemical staining of consecutive tumor sections from M1 (A-D) , M2 (E-H) and M3 (I-L) tumor xenografts using hematoxylin and eosin (A, E & I) , anti-human transferrin (Tf) (B, F & J) , anti-human transferrin receptor (TfR) (C, G & K) and anti-glucose transporter 1 (GLUT1) (D, H & I) . NovaRED was used as peroxidase substrate (brown color), sections were counterstained with methyl green. White dashed lines delineate necrotic core (NC). Scale bar = 100 um.

GLUT1 Antibody — FIGURE 3 Western blot analysis bar charts show differences in Glut1 (~55 kDa) protein expression in cortical (A) and subcortical (D) brain tissue at the end of the 32-wk diet treatment. Representative blots of cortical (B) and subcortical (E) brain tissue with actin reprobes are shown. Deglycosylation evaluation blots of cortical (C) and subcortical (F) brain tissue show nondeglycosylated control (1) and deglycosylated (2) samples (41% lard). Values are means +- SEMs, n = 11-12/group. Two-factor ANOVA with Tukey-Kramer post hoc test was conducted when interactions were significant. *Different from 10% lard, P < 0.05. Glut-1, glucose-1 transporter Slc2a1. {TM}

Product Details

PA1-46152

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	View 9 publications 9 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	1:200	-
Immunocytochemistry (ICC/IF)	1:1,000	View 1 publication 1 publication
Flow Cytometry (Flow)	Assay-Dependent	-
ChIP assay (ChIP)	Assay-Dependent	-
In vitro Assay (IV)	Assay-Dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Hamster, Human, Mouse, Rabbit
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Human GLUT1 protein (between residues 1-100). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	-20°C or -80°C if preferred
RRID	AB_2302087

Product Specific Information

The target sequence has 100% sequence homology with primate, and 93% with rat, bovine, and rabbit.

Target Information

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of lamprey variable lymphocyte receptors that target the brain vasculature.

Scientific reports

Lajoie JM,Katt ME,Waters EA,Herrin BR,Shusta EV

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Mon Apr 11 00:00:00 EDT 2022

Synthetic adiponectin-receptor agonist, AdipoRon, induces glycolytic dependence in pancreatic cancer cells.

Cell death & disease

Manley SJ,Olou AA,Jack JL,Ruckert MT,Walsh RM,Eades AE,Bye BA,Ambrose J,Messaggio F,Anant S,VanSaun MN

PA1-46152 was used in Western Blot to provide evidence that pancreatic cancer cells utilize metabolic plasticity to upregulate glycolysis in order to adapt to suppression of oxidative phosphorylation in the presence of AdipoRon.

Fri Feb 04 00:00:00 EST 2022

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.

Theranostics

Rudkouskaya A,Sinsuebphon N,Ochoa M,Chen SJ,Mazurkiewicz JE,Intes X,Barroso M

PA1-46152 was used in Immunohistochemistry to enable simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

Mon May 24 00:00:00 EDT 2021

Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.

Theranostics

Rudkouskaya A,Sinsuebphon N,Ochoa M,Chen SJ,Mazurkiewicz JE,Intes X,Barroso M

PA1-46152 was used in Immunohistochemistry to enable simultaneous non-invasive monitoring of intracellular drug delivery and metabolic response in preclinical studies.

Mon May 24 00:00:00 EDT 2021

Free fatty acids receptors 2 and 3 control cell proliferation by regulating cellular glucose uptake.

World journal of gastrointestinal oncology

Al Mahri S,Al Ghamdi A,Akiel M,Al Aujan M,Mohammad S,Aziz MA

PA1-46152 was used in Immunocytochemistry-immunoflourescence to understand the role of short chain free fatty acid receptors (FFARs) in colorectal cancer (CRC).

Fri May 15 00:00:00 EDT 2020

Extracellular pH mapping of liver cancer on a clinical 3T MRI scanner.

Magnetic resonance in medicine

Coman D,Peters DC,Walsh JJ,Savic LJ,Huber S,Sinusas AJ,Lin M,Chapiro J,Constable RT,Rothman DL,Duncan JS,Hyder F

PA1-46152 was used in Immunohistochemistry to demonstrate feasibility and potential clinical translatability of high-resolution pHe mapping to monitor tumor aggressiveness and therapeutic outcome, all to improve personalized cancer treatment planning.

Fri May 01 00:00:00 EDT 2020

Molecular Imaging of Extracellular Tumor pH to Reveal Effects of Locoregional Therapy on Liver Cancer Microenvironment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Savic LJ,Schobert IT,Peters D,Walsh JJ,Laage-Gaupp FM,Hamm CA,Tritz N,Doemel LA,Lin M,Sinusas A,Schlachter T,Duncan JS,Hyder F,Coman D,Chapiro J

PA1-46152 was used in Immunohistochemistry to establish magnetic resonance (MR)-based molecular imaging paradigms for the noninvasive monitoring of extracellular pH (pHe) as a functional surrogate biomarker for metabolic changes induced by locoregional therapy of liver cancer.

Wed Jan 15 00:00:00 EST 2020

AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized Fe₃O₄ nanoparticles during oxidant-induced senescence in human fibroblasts.

Redox biology

Lewinska A,Adamczyk-Grochala J,Bloniarz D,Olszowka J,Kulpa-Greszta M,Litwinienko G,Tomaszewska A,Wnuk M,Pazik R

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Wed Jan 01 00:00:00 EST 2020

Acyl-CoA-Binding Protein Is a Lipogenic Factor that Triggers Food Intake and Obesity.

Cell metabolism

Bravo-San Pedro JM,Sica V,Martins I,Pol J,Loos F,Maiuri MC,Durand S,Bossut N,Aprahamian F,Anagnostopoulos G,Niso-Santano M,Aranda F,Ramírez-Pardo I,Lallement J,Denom J,Boedec E,Gorwood P,Ramoz N,Clément K,Pelloux V,Rohia A,Pattou F,Raverdy V,Caiazzo R,Denis RGP,Boya P,Galluzzi L,Madeo F,Migrenne-Li S,Cruciani-Guglielmacci C,Tavernarakis N,López-Otín C,Magnan C,Kroemer G

PA1-46152 was used in Western Blotting to design three approaches to neutralize ACBP, namely, inducible whole-body knockout, systemic administration of neutralizing antibodies, and induction of antiACBP autoantibodies in mice.

Tue Oct 01 00:00:00 EDT 2019

Interactions between TGF-β type I receptor and hypoxia-inducible factor-α mediates a synergistic crosstalk leading to poor prognosis for patients with clear cell renal cell carcinoma.

Cell cycle (Georgetown, Tex.)

Mallikarjuna P,Raviprakash TS,Aripaka K,Ljungberg B,Landström M

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Sun Sep 01 00:00:00 EDT 2019

HIF-1α regulates IL-1β and IL-17 in sarcoidosis.

eLife

Talreja J,Talwar H,Bauerfeld C,Grossman LI,Zhang K,Tranchida P,Samavati L

PA1-46152 was used in Western Blotting to suggest that increased activity of HIF-α isoforms regulate Th1/Th17 mediated inflammation in sarcoidosis.

Wed May 01 00:00:00 EDT 2019

Mfsd2a and Glut1 Brain Nutrient Transporters Expression Increase with 32-Week Low and High Lard Compared with Fish-Oil Dietary Treatment in C57Bl/6 Mice.

Current developments in nutrition

Sandoval KE,Wooten JS,Harris MP,Schaller ML,Umbaugh DS,Witt KA

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Mon Oct 01 00:00:00 EDT 2018

Photoperiodic modulation of thyroid hormone receptor (TR-α), deiodinase-2 (Dio-2) and glucose transporters (GLUT 1 and GLUT 4) expression in testis of adult golden hamster, Mesocricetus auratus.

Journal of photochemistry and photobiology. B, Biology

Verma R,Haldar C

PA146152 was used in western blot to propose that testicular thyroid hormone status is regulated by photoperiod in golden hamsters

Thu Dec 01 00:00:00 EST 2016

Somatic gain-of-function HIF2A mutations in sporadic central nervous system hemangioblastomas.

Journal of neuro-oncology

Taïeb D,Barlier A,Yang C,Pertuit M,Tchoghandjian A,Rochette C,Zattara-Canoni H,Figarella-Branger D,Zhuang Z,Pacak K,Metellus P

PA1-46152 was used in immunohistochemistry - paraffin section to examine the association between central nervous system hemangioblastomas and HIF2A mutations

Mon Feb 01 00:00:00 EST 2016

N-3PUFA differentially modulate palmitate-induced lipotoxicity through alterations of its metabolism in C2C12 muscle cells.

Biochimica et biophysica acta

Pinel A,Rigaudière JP,Laillet B,Pouyet C,Malpuech-Brugère C,Prip-Buus C,Morio B,Capel F

PA1-46152 was used in western blot to assess the effects of n-3PUFA on fatty acid metabolism and insulin sensitivity in muscle cells

Fri Jan 01 00:00:00 EST 2016

Metabolism modifications and apoptosis induction after Cellfood™ administration to leukemia cell lines.

Journal of experimental & clinical cancer research : CR

Catalani S,Carbonaro V,Palma F,Arshakyan M,Galati R,Nuvoli B,Battistelli S,Canestrari F,Benedetti S

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Mon Sep 09 00:00:00 EDT 2013

Metabolism modifications and apoptosis induction after Cellfood™ administration to leukemia cell lines.

Journal of experimental & clinical cancer research : CR

Catalani S,Carbonaro V,Palma F,Arshakyan M,Galati R,Nuvoli B,Battistelli S,Canestrari F,Benedetti S

Published figure using GLUT1 polyclonal antibody (Product # PA1-46152) in Western Blot

Mon Sep 09 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

Function(s)

protein complex assembly transport carbohydrate transport glucose transport proton transport hexose transmembrane transport cellular response to glucose starvation xenobiotic transport glucose import transmembrane transport dehydroascorbic acid transport cation transmembrane transport lactose biosynthetic process response to osmotic stress L-ascorbic acid metabolic process regulation of insulin secretion glucose transmembrane transport

Process(es)

glucose transmembrane transporter activity protein binding kinase binding dehydroascorbic acid transporter activity identical protein binding xenobiotic transporter activity protein self-association D-glucose transmembrane transporter activity transporter activity sugar:proton symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

GLUT1 Polyclonal Antibody

Product Details

PA1-46152

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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