GLUT1 Polyclonal Antibody

Product Specific Information

This antibody is predicted to react with bovine, chicken, mouse, porcine, rabbit and rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is esophageal or breast carcinoma.

Target Information

Glucose transporter 1 (GLUT1), encoded by the SLC2A1 gene, is a facilitative glucose transporter that plays a pivotal role in the uptake of glucose across cell membranes, particularly in tissues with high-energy demands such as the brain, erythrocytes, and endothelial cells of the blood-brain barrier. GLUT1 is ubiquitously expressed and functions through a passive diffusion mechanism, allowing glucose to move down its concentration gradient. Its involvement in maintaining glucose homeostasis is crucial for cellular metabolism and energy production. Mutations in SLC2A1 can lead to GLUT1 deficiency syndrome, characterized by seizures, developmental delay, and movement disorders due to impaired glucose transport into the brain. GLUT1 also plays a significant role in cancer biology, where its overexpression is commonly observed in various tumors, facilitating the enhanced glucose uptake required for rapid tumor cell proliferation. This characteristic makes GLUT1 a potential target for cancer diagnostics and therapeutics. Additionally, GLUT1's regulation is influenced by factors such as hypoxia, insulin, and cellular stress, highlighting its adaptive role in different physiological and pathological states.