Dissected inner ear tissues from mice were immersed in 20% sucrose PBS solution (pH 7.4) overnight followed by being embedded in OCT overnight. The tissues were snap frozen in liquid nitrogen and 7um sections were cut by cryostat. Cochlear sections from the organ of Corti were dissected and rinsed by 0.1% Triton in PBS (pH 7.4, PBST) for 30 minutes. Sections were blocked with 5% goat serum in PBST at room temperature for 1 hour. The GLUT10 antibody was used at a 1:200 dilution to label the cochlear sections at 4 degrees C overnight. After washing, the sections were incubated with secondary antibodies (anti-rabbit Cy3, 1:400) at room temperature for 1 hour. Cy3-conjugated secondary antibodies were used to visualize the binding of the primary antibodies. The slides were mounted with fluoromont G and examined under a confocal microscope. Data from cochlear sections show strong immunoreactivity (stained red) is observed in the inner hair cells, outer hair cells and basilar membrane. In addition, spiral ganglion neurons display moderate staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Residues 367-385 of the human GLUT10 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Non-insulin-dependent diabetes mellitus (NIDDM) is a multifactoral disease with both environmental and genetics causes. Genome-wide screening procedures have identified several susceptibility loci for NIDDM within the human genome. A putative sugar transp
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.