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Invitrogen
GLUT4 Polyclonal Antibody
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FIGURE: 1 / 18
GLUT4 Antibody (PA5-23052) in ICC/IF
Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase 3T3-L1 cells differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT4 Rabbit Polyclonal Antibody (Product # PA5-23052) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-23052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat
Published species
Hamster,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from the human Glucose Transporter GLUT4 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with rat based on 100% sequence homology.
Target Information
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glucose transporter 2; glucose transporter 4 insulin-responsive; Glucose transporter 4, insuline-responsive; glucose transporter glut4; glucose transporter type 4, insulin-responsive; GLUT-4; insulin responsive glucose transporter; insulin-responsive glucose transporter; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2 , member 4; unnamed protein product
Gene Aliases: Glut-4; GLUT4; GT2; twgy
UniProt ID: (Human) P14672, (Mouse) P14142, (Rat) P19357
Entrez Gene ID: (Human) 6517, (Mouse) 20528, (Rat) 25139
learning or memory
short-term memory
long-term memory
amylopectin biosynthetic process
positive regulation of brain-derived neurotrophic factor receptor signaling pathway
cellular response to insulin stimulus
glucose homeostasis
glucose import in response to insulin stimulus
response to ethanol
brown fat cell differentiation
transmembrane transport
dehydroascorbic acid transport
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to osmotic stress
regulation of synaptic vesicle budding from presynaptic endocytic zone membrane
glucose import across plasma membrane
glucose transmembrane transport
carbohydrate metabolic process
white fat cell proliferation
transport across blood-brain barrier
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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