Invitrogen

GLUT4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 Published Figures

17 References

View all (30) GLUT4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GLUT4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

GLUT4 Antibody (PA5-23052) in ICC/IF

Immunofluorescence analysis of GLUT4 was performed using 70% confluent log phase 3T3-L1 cells differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GLUT4 Rabbit Polyclonal Antibody (Product # PA5-23052) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More

GLUT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GLUT4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

GLUT4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using GLUT4 Rabbit Polyclonal Antibody (Product # PA5-23052) showed expression of GLUT4 only in 3T3-L1 cells differentiated to adipocytes as compared to undifferentiated 3T3-L1 cells. {RE}

Product Details

PA5-23052

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

Assay-Dependent

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Plant, Rat

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the human Glucose Transporter GLUT4 protein (between amino acids 1-100) [UniProt P14672]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153908

Product Specific Information

This antibody is predicted to react with rat based on 100% sequence homology.

Target Information

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BPS-induced ovarian dysfunction: Protective actions of melatonin via modulation of SIRT-1/Nrf2/NFĸB and IR/PI3K/pAkt/GLUT-4 expressions in adult golden hamster.

Journal of pineal research

Pal S,Sahu A,Verma R,Haldar C

Published figure using GLUT4 polyclonal antibody (Product # PA5-23052) in Western Blot

Tue Aug 01 00:00:00 UTC 2023

Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle.

eLife

Knudsen JR,Persson KW,Henriquez-Olguin C,Li Z,Di Leo N,Hesselager SA,Raun SH,Hingst JR,Trouillon R,Wohlwend M,Wojtaszewski JFP,Gijs MAM,Jensen TE

PA5-23052 was used in Immunohistochemistry to study microtubule-based GLUT4 trafficking in human and mouse muscle fibers and L6 rat muscle cells.

Wed Apr 19 00:00:00 UTC 2023

The Effect of Lower Limb Combined Neuromuscular Electrical Stimulation on Skeletal Muscle Signaling for Glucose Utilization, Myofiber Distribution, and Metabolic Function after Spinal Cord Injury.

International journal of environmental research and public health

Alharbi A,Li J,Womack E,Farrow M,Yarar-Fisher C

PA5-23052 was used in Immunohistochemistry to investigate if maintaining healthy myofiber type and metabolic function may be achieved via early utilization of combined (aerobic + resistance) neuromuscular electrical stimulation.

Sat Oct 21 00:00:00 UTC 2023

The Effect of Lower Limb Combined Neuromuscular Electrical Stimulation on Skeletal Muscle Signaling for Glucose Utilization, Myofiber Distribution, and Metabolic Function after Spinal Cord Injury.

International journal of environmental research and public health

Alharbi A,Li J,Womack E,Farrow M,Yarar-Fisher C

Sat Oct 21 00:00:00 UTC 2023

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Genes

Liu Y,Wang Y,Yao D,Chen X,Zhang F,Feng Y,Li X

PA5-23052 was used in Immunohistochemistry (Paraffin), Western Blot to support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Wed Jan 12 00:00:00 UTC 2022

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Genes

Liu Y,Wang Y,Yao D,Chen X,Zhang F,Feng Y,Li X

PA5-23052 was used in Immunohistochemistry (Paraffin), Western Blot to support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Wed Jan 12 00:00:00 UTC 2022

Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.

Genes

Liu Y,Wang Y,Yao D,Chen X,Zhang F,Feng Y,Li X

PA5-23052 was used in Immunohistochemistry (Paraffin), Western Blot to support the use of Diane-35 and metformin combination therapy for patients with PCOS and early EC.

Wed Jan 12 00:00:00 UTC 2022

Resistance exercise training-induced skeletal muscle strength provides protective effects on high-fat-diet-induced metabolic stress in mice.

Laboratory animal research

Kim HJ,Kim YJ,Kim IY,Seong JK

PA5-23052 was used in Immunohistochemistry to evaluate whether the improvement in skeletal muscle strength and endurance by training were correlated with improved metabolism.

Fri Dec 02 00:00:00 UTC 2022

Resistance exercise training-induced skeletal muscle strength provides protective effects on high-fat-diet-induced metabolic stress in mice.

Laboratory animal research

Kim HJ,Kim YJ,Kim IY,Seong JK

PA5-23052 was used in Immunohistochemistry to evaluate whether the improvement in skeletal muscle strength and endurance by training were correlated with improved metabolism.

Fri Dec 02 00:00:00 UTC 2022

Resistance exercise training-induced skeletal muscle strength provides protective effects on high-fat-diet-induced metabolic stress in mice.

Laboratory animal research

Kim HJ,Kim YJ,Kim IY,Seong JK

PA5-23052 was used in Immunohistochemistry to evaluate whether the improvement in skeletal muscle strength and endurance by training were correlated with improved metabolism.

Fri Dec 02 00:00:00 UTC 2022

Clenbuterol exerts antidiabetic activity through metabolic reprogramming of skeletal muscle cells.

Nature communications

Meister J,Bone DBJ,Knudsen JR,Barella LF,Velenosi TJ,Akhmedov D,Lee RJ,Cohen AH,Gavrilova O,Cui Y,Karsenty G,Chen M,Weinstein LS,Kleinert M,Berdeaux R,Jensen TE,Richter EA,Wess J

PA5-23052 was used in Immunohistochemistry to elucidate the underlying cellular and molecular mechanisms of the beneficial metabolic effects of systemic treatment with β2-adrenergic receptor agonists.

Mon Jan 10 00:00:00 UTC 2022

Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness.

Cancers

Tossetta G,Fantone S,Gesuita R,Goteri G,Senzacqua M,Marcheggiani F,Tiano L,Marzioni D,Mazzucchelli R

PA5-23052 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that CNTF is expressed in PCa and CRPC tissues, and has a pivotal role in prostate cancer environment remodeling and as a negative modulator of invasion processes of CRPC cell models.

Wed Nov 30 00:00:00 UTC 2022

Dexmedetomidine reduces myocardial ischemia-reperfusion injury in young mice through MIF/AMPK/GLUT4 axis.

BMC anesthesiology

Chen S,Li A,Wu J,Huang Y,Zou T,Tailaiti T,Wang J

PA5-23052 was used in Western Blotting to reveal the consequences of Dex on myocardial ischemia-reperfusion injury (IRI) in young mice.

Wed Sep 14 00:00:00 UTC 2022

CXCR1/2 Inhibitor Ladarixin Ameliorates the Insulin Resistance of 3T3-L1 Adipocytes by Inhibiting Inflammation and Improving Insulin Signaling.

Cells

Castelli V,Brandolini L,d'Angelo M,Giorgio C,Alfonsetti M,Cocchiaro P,Lombardi F,Cimini A,Allegretti M

Published figure using GLUT4 polyclonal antibody (Product # PA5-23052) in Western Blot

Mon Sep 06 00:00:00 UTC 2021

Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation.

Molecular metabolism

Knudsen JR,Steenberg DE,Hingst JR,Hodgson LR,Henriquez-Olguin C,Li Z,Kiens B,Richter EA,Wojtaszewski JFP,Verkade P,Jensen TE

PA5-23052 was used in Immunohistochemistry to reveal that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli.

Tue Sep 01 00:00:00 UTC 2020

Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation.

Molecular metabolism

Knudsen JR,Steenberg DE,Hingst JR,Hodgson LR,Henriquez-Olguin C,Li Z,Kiens B,Richter EA,Wojtaszewski JFP,Verkade P,Jensen TE

PA5-23052 was used in Immunohistochemistry to reveal that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli.

Tue Sep 01 00:00:00 UTC 2020

Photoperiodic modulation of thyroid hormone receptor (TR-α), deiodinase-2 (Dio-2) and glucose transporters (GLUT 1 and GLUT 4) expression in testis of adult golden hamster, Mesocricetus auratus.

Journal of photochemistry and photobiology. B, Biology

Verma R,Haldar C

PA523052 was used in western blot to propose that testicular thyroid hormone status is regulated by photoperiod in golden hamsters

Thu Dec 01 00:00:00 UTC 2016

Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice.

The Journal of physiology

Sylow L,Nielsen IL,Kleinert M,Møller LL,Ploug T,Schjerling P,Bilan PJ,Klip A,Jensen TE,Richter EA

PA5-23052 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation.

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Glucose transporter 4 insuline-responsive; Glucose transporter 4, insuline-responsive; glucose transporter glut4; Glucose transporter type 4, insulin-responsive; GLUT-4; GT2; insulin responsive glucose transporter; insulin-responsive glucose transporter type 4; MGC93607; solute carrier family 2 member 4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2 , member 4; Solute carrier family 2, facilitated glucose transporter member 4

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Gene Aliases: Glut-4; GLUT4; SLC2A4

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UniProt ID: (Human) P14672, (Rat) P19357, (Mouse) P14142

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Entrez Gene ID: (Human) 6517, (Rat) 25139, (Mouse) 20528

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Function(s)

glucose transmembrane transporter activity protein binding D-glucose transmembrane transporter activity transporter activity insulin-responsive hydrogen:glucose symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

Process(es)

amylopectin biosynthetic process glucose transport cellular response to insulin stimulus hexose transmembrane transport glucose homeostasis response to ethanol brown fat cell differentiation transmembrane transport cellular response to hypoxia cellular response to osmotic stress carbohydrate metabolic process glucose import glucose transmembrane transport transport carbohydrate transport proton transport cation transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GLUT4 Polyclonal Antibody

GLUT4 Antibody (PA5-23052) in ICC/IF

Product Details

PA5-23052

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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