Immunofluorescent analysis of GLYCTK showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a GLYCTK polyclonal antibody (Product # PA5-28660) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 249 and 523 of Human GLYCTK|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28660 targets GLYCTK in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28660 immunogen is recombinant fragment corresponding to a region within amino acids 249 and 523 of Human GLYCTK.
This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described.
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