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Invitrogen
GNAI1 Monoclonal Antibody (R4.5)
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FIGURE: 1 / 5
GNAI1 Antibody (MA5-12800) in ICC/IF
Immunofluorescence analysis of Galphai1 (GNAI1) was performed using 70% confluent log phase RSC-96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GNAI1 (R4.5) Mouse Monoclonal Antibody (Product # MA5-12800) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
Product Details
MA5-12800
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
R4.5
Immunogen
Partially purified rat brain Galphai1 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12800 targets Galphai1 G-Protein in WB applications and shows reactivity with Bovine, Guinea Pig, Human, mouse, and Rat samples.
The MA5-12800 immunogen is partially purified rat brain Galphai1 protein.
Target Information
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain; and transducin-1 and transducin-2, proteins involved in phototransduction in retinal rods and cones, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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