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Invitrogen
GNAI3 Polyclonal Antibody
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FIGURE: 1 / 1
GNAI3 Antibody (PA5-120385) in WB
Western blot analysis of GNAI3 in extracts of various cell lines. Samples were incubated with GNAI3 Polyclonal antibody (Product # PA5-120385) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120385
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).
Conjugate
Form
Liquid
Concentration
0.86 mg/mL
Amount
86 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: LO2, HepG2, Mouse kidney, Mouse brain, Rat kidney. The target is usually found in the following locations: Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence: MGCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV KQMKIIHEDG YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR
Target Information
Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i. e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13. The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust. Of these, the three Galpha i subtypes function to open atrial potassium channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein; G protein alpha subunit; G(i) alpha-3; GTP-binding protein alpha subunit; guanine nucleotide binding protein (G protein), alpha inhibiting 3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide binding protein, alpha inhibiting 3; Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3; guanine nucleotide-binding protein (G) alpha subunit; guanine nucleotide-binding protein G(i) subunit alpha; Guanine nucleotide-binding protein G(i) subunit alpha-3; guanine nucleotide-binding protein G(k) subunit alpha; guanine nucleotide-binding regulatory protein alpha-inhibitory subunit; heterotrimeric guanine nucleotide-binding protein 1A; unnamed protein product
Gene Aliases: 87U6; AI158965; ARCND1; ARCODS; AW537698; Galphai3; GIP3A; Gnai-3; GNAI3; HG1A
UniProt ID: (Human) P08754, (Rat) P08753, (Mouse) Q9DC51
Entrez Gene ID: (Human) 2773, (Rat) 25643, (Mouse) 14679
nucleotide binding
G-protein coupled receptor binding
GTPase activity
protein binding
GTP binding
guanyl nucleotide binding
GDP binding
G-protein beta/gamma-subunit complex binding
metal ion binding
signal transducer activity
protein domain specific binding
G-protein coupled serotonin receptor binding
GTPase activating protein binding
heterotrimeric G-protein
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
negative regulation of adenylate cyclase activity
positive regulation of macroautophagy
GTP metabolic process
cell division
vesicle fusion
regulation of heart contraction
metabolic process
negative regulation of secretion
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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