Invitrogen

GNAL Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

GNAL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — GNAL Polyclonal Antibody detects GNAL protein at cytosol on NCI-N87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded NCI-N87 xenograft. GNAL Polyclonal Antibody (Product # PA5-27964) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

GNAL Antibody in Western Blot (WB) — Western Blot using GNAL Polyclonal Antibody (Product # PA5-27964). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL Polyclonal Antibody (Product # PA5-27964) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-27964

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 122 and 381 of Human GNAL View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.65 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545440

Product Specific Information

Recommended positive controls: Raji, mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (92%), Chicken (95%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impaired dopamine- and adenosine-mediated signaling and plasticity in a novel rodent model for DYT25 dystonia.

Neurobiology of disease

Yu-Taeger L,Ott T,Bonsi P,Tomczak C,Wassouf Z,Martella G,Sciamanna G,Imbriani P,Ponterio G,Tassone A,Schulze-Hentrich JM,Goodchild R,Riess O,Pisani A,Grundmann-Hauser K,Nguyen HP

PA5-27964 was used in Western Blot to generate and characterise heterozygous Gnal knockout rats (Gnal+/ - ) with a 13 base pair deletion in the first exon of the rat Gnal splicing variant 2.

Sat Feb 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Adenylate cyclase-stimulating G alpha protein, olfactory type; G alpha 10; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type; guanine nucleotide binding protein, alpha 10; Guanine nucleotide-binding protein G(olf) subunit alpha; olfa

Gene Aliases: 2610011C15Rik; 9630020G10Rik; DYT25; Galphaolf; Gna10; GNAL; Golf

UniProt ID: (Human) P38405, (Mouse) Q8CGK7

Entrez Gene ID: (Human) 2774, (Mouse) 14680

Molecular Function: heterotrimeric G-protein