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GNAO1 Antibody (PA5-26142) in WB
Western blot analysis of GNAO1 in various lysates. Samples were incubated with GNAO1 polyclonal antibody (Product # PA5-26142) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Mouse cerebellum whole lysate; Lane 2: Mouse brain whole lysate; Lane 3: Rat brain whole lysate. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-26142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 291-320 amino acids from the C-terminal region of human GNAO1
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2543642
Product Specific Information
This antibody is predicted to react with bovine based on sequence homology.
Target Information
Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686O0962; G alpha o; GNAO 1; Go alpha; GO2-q chimeric G-protein; GTP-binding protein alpha o; GTP-binding protein alpha-o subunit; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha O polypeptide 1; Guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding regulatory protein 2
Gene Aliases: alphaO; AW050213; EIEE17; G-ALPHA-o; Galphao; Gna0; GNAO; GNAO1; HLA-DQB1; RATBPGTPC
UniProt ID: (Human) P09471, (Rat) P59215, (Mouse) P18872
Entrez Gene ID: (Human) 2775, (Rat) 50664, (Mouse) 14681
GTPase activity
signal transducer activity
GTP binding
G-protein beta/gamma-subunit complex binding
G-protein coupled serotonin receptor binding
mu-type opioid receptor binding
GTPase activating protein binding
metal ion binding
corticotropin-releasing hormone receptor 1 binding
protein binding
GDP binding
protein complex binding
nucleotide binding
guanyl nucleotide binding
heterotrimeric G-protein
protein folding
muscle contraction
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
dopamine receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
aging
locomotory behavior
regulation of heart contraction
forebrain development
neuron projection development
response to cytokine
response to drug
response to hydrogen peroxide
response to morphine
positive regulation of GTPase activity
negative regulation of calcium ion transport
G-protein coupled receptor signaling pathway
metabolic process
cellular process
response to organonitrogen compound
response to organic cyclic compound
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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