Search
Invitrogen
GNAS Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
GNAS Antibody (PA5-98353) in ICC/IF
Immunofluorescent analysis of GNAS in MCF-7 cells using a GNAS polyclonal antibody (Product # PA5-98353) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-98353
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Protein ALEX protein (310-404AA)
Conjugate
Form
Liquid
Concentration
3.04 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Mutations in GNAS gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseous heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. This gene has a highly complex imprinted expression pattern. It encodes maternally, paternally, and biallelically expressed proteins which are derived from alternatively spliced transcripts with alternate 5' exons. Each of the upstream exons is within a differentially methylated region, commonly found in imprinted genes. However, the close proximity (14 kb) of two oppositely expressed promoter regions is unusual. In addition, one of the alternate 5' exons introduces a frameshift relative to the other transcripts, resulting in one isoform which is structurally unrelated to the others. An antisense transcript exists, and may regulate imprinting in this region. Mutations in this gene result in pseudohypoparathyroidism type 1a (PHP1a), which has an atypical autosomal dominant inheritance pattern requiring maternal transmission for full penetrance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Adenylate cyclase-stimulating G alpha protein; Alternative gene product encoded by XL-exon; dJ309F20.1.1; dJ806M20.3.3; Extra large alphas protein; G protein; G protein subunit alpha S; GNSA1; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1; guanine nucleotide regulatory protein; guanine nucleotide-binding protein alpha subunit; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; MGC33735; NESP55; NESP55; large dense core vesicle secretory protein; Neuroendocrine secretory protein 55; OTTHUMP00000031742; OTTHUMP00000196026; OTTHUMP00000196030; PHP1C; Protein ALEX; RP4-543J19.4; secretogranin VI; unnamed protein product; XLalphas; XLas
Gene Aliases: AHO; AIMAH1; C20orf45; GNAS; GNAS1; GPSA; GSA; GSP; NESP; PITA3; POH; SCG6; SgVI
UniProt ID: (Human) Q5JWF2
Entrez Gene ID: (Human) 2778
nucleotide binding
GTPase activity
obsolete small monomeric GTPase activity
insulin-like growth factor receptor binding
protein binding
GTP binding
adenylate cyclase regulator activity
adenylate cyclase activator activity
hydrolase activity
guanyl nucleotide binding
G-protein beta/gamma-subunit complex binding
beta-2 adrenergic receptor binding
D1 dopamine receptor binding
mu-type opioid receptor binding
ionotropic glutamate receptor binding
metal ion binding
corticotropin-releasing hormone receptor 1 binding
negative regulation of inflammatory response to antigenic stimulus
renal water homeostasis
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
adenylate cyclase-activating dopamine receptor signaling pathway
adenylate cyclase-activating serotonin receptor signaling pathway
female pregnancy
sensory perception of chemical stimulus
sensory perception of smell
protein secretion
regulation of signal transduction
regulation of skeletal muscle contraction
response to prostaglandin E
negative regulation of multicellular organism growth
intracellular transport
developmental growth
positive regulation of phagocytosis
regulation of insulin secretion
cognition
bone development
hair follicle placode formation
platelet aggregation
response to parathyroid hormone
cellular response to glucagon stimulus
cellular response to prostaglandin E stimulus
cellular response to acidic pH
cellular response to catecholamine stimulus
adenylate cyclase-activating adrenergic receptor signaling pathway
vascular endothelial cell response to laminar fluid shear stress
positive regulation of cold-induced thermogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support