Recommended positive controls: Jurkat, Raji, NCI-H929, Mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta subunit, signal-transducing proteins GS/GI; gbb1; guanine nucleotide binding protein (G protein), beta 1; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide binding protein, beta 1; Guanine nucleotide-binding protein beta 1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein, beta-1 subunit; T beta; testicular tissue protein Li 72; Transducin beta chain 1
Gene Aliases: AA409223; C77571; Gnb-1; GNB1; MRD42