Proteintech

GNMT Polyclonal Antibody

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GNMT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP ( GNMT antibody) at dilution of 1:200 (under 40x lens).

GNMT Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GNMT Antibody in Immunoprecipitation (IP) — IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:600) with mouse liver tissue lysate 6800ug.

Product Details

18790-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 12 publications 12 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Chicken, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GNMT Fusion Protein Ag4598 (1-295 aa encoded by BC032627) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.36 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MVDSVYRTR SLGVAAEGLP DQYADGEAAR VWQLYIGDTR SRTAEYKAWL LGLLRQHGCQ RVLDVACGTG VDSIMLVEEG FSVTSVDASD KMLKYALKER WNRRHEPAFD KWVIEEANWM TLDKDVPQSA EGGFDAVICL GNSFAHLPDC KGDQSEHRLA LKNIASMVRA GGLLVIDHRN YDHILSTGCA PPGKNIYYKS DLTKDVTTSV LIVNNKAHMV TLDYTVQVPG AGQDGSPGLS KFRLSYYPHC LASFTELLQA AFGGKCQHSV LGDFKPYKPG QTYIPCYFIH VLKRTD (1-295 aa encoded by B C032627)

Target Information

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dietary betaine activates hepatic VTGII expression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression.

Journal of animal science and biotechnology

Omer NA,Hu Y,Hu Y,Idriss AA,Abobaker H,Hou Z,Dong H,Zhao R

Fri Mar 11 00:00:00 EST 2022

Homocysteine impairs porcine oocyte quality via deregulation of one-carbon metabolism and hypermethylation of mitochondrial DNA†.

Biology of reproduction

Jia L,Zeng Y,Hu Y,Liu J,Yin C,Niu Y,Wang C,Li J,Jia Y,Hong J,Zhao R

Mon Apr 01 00:00:00 EDT 2019

Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.

The Journal of nutritional biochemistry

Zhao N,Yang S,Jia Y,Sun B,He B,Zhao R

Sun Apr 01 00:00:00 EDT 2018

Mitotically Stable Modification of DNA Methylation in IGF2/H19 Imprinting Control Region Is Associated with Activated Hepatic IGF2 Expression in Offspring Rats from Betaine-Supplemented Dams.

Journal of agricultural and food chemistry

Yang S,Zhao N,Yang Y,Hu Y,Dong H,Zhao R

Wed Mar 21 00:00:00 EDT 2018

Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation.

The Journal of nutrition

Hu Y,Sun Q,Hu Y,Hou Z,Zong Y,Omer NA,Abobaker H,Zhao R

Thu Mar 01 00:00:00 EST 2018

Maternal betaine supplementation in rats induces intergenerational changes in hepatic IGF-1 expression and DNA methylation.

Molecular nutrition & food research

Zhao N,Yang S,Hu Y,Dong H,Zhao R

Tue Aug 01 00:00:00 EDT 2017

Maternal Betaine Supplementation throughout Gestation and Lactation Modifies Hepatic Cholesterol Metabolic Genes in Weaning Piglets via AMPK/LXR-Mediated Pathway and Histone Modification.

Nutrients

Cai D,Yuan M,Liu H,Pan S,Ma W,Hong J,Zhao R

Tue Oct 18 00:00:00 EDT 2016

Downregulation of miR-150 Expression by DNA Hypermethylation Is Associated with High 2-Hydroxy-(4-methylthio)butanoic Acid-Induced Hepatic Cholesterol Accumulation in Nursery Piglets.

Journal of agricultural and food chemistry

Jia Y,Ling M,Zhang L,Jiang S,Sha Y,Zhao R

Wed Oct 12 00:00:00 EDT 2016

Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.

The international journal of biochemistry & cell biology

Yang Y,Li X,Sun Q,He B,Jia Y,Cai D,Zhao R

Sat Oct 01 00:00:00 EDT 2016

Abnormally activated one-carbon metabolic pathway is associated with mtDNA hypermethylation and mitochondrial malfunction in the oocytes of polycystic gilt ovaries.

Scientific reports

Jia L,Li J,He B,Jia Y,Niu Y,Wang C,Zhao R

Wed Jan 13 00:00:00 EST 2016

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Journal of agricultural and food chemistry

Jia Y,Song H,Gao G,Cai D,Yang X,Zhao R

Wed Nov 25 00:00:00 EST 2015

Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions.

European journal of nutrition

Li X,Sun Q,Li X,Cai D,Sui S,Jia Y,Song H,Zhao R

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 2.1.1.20; epididymis secretory sperm binding protein Li 182mP; Folate-binding protein; glycine methyltransferase; glycine N methyl transferase; Glycine N-methyltransferase

Gene Aliases: Fbp-cII; GNMT; HEL-S-182mP

UniProt ID: (Human) Q14749, (Mouse) Q9QXF8, (Rat) P13255

Entrez Gene ID: (Human) 27232, (Mouse) 14711, (Rat) 25134

Molecular Function: methyltransferase