Proteintech

GNPAT Polyclonal Antibody

Product Image Gallery

GNPAT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody 14931-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GNPAT Antibody in Western Blot (WB) — Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GNPAT Antibody in Immunoprecipitation (IP) — IP result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

Product Details

14931-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Hamster, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GNPAT Fusion Protein Ag6735 (331-680 aa encoded by BC000450) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody recognize the 69 kDa GNPAT protein.

Immunogen sequence: PVSLRSLAA GRMSRSSYNL VPRYIPQKQS EDMHAFVTEV AYKMELLQIE NMVLSPWTLI VAVLLQNRPS MDFDALVEKT LWLKGLTQAF GGFLIWPDNK PAEEVVPASI LLHSNIASLV KDQVILKVDS GDSEVVDGLM LQHITLLMCS AYRNQLLNIF VRPSLVAVAL QMTPGFRKED VYSCFRFLRD VFADEFIFLP GNTLKDFEEG CYLLCKSEAI QVTTKDILVT EKGNTVLEFL VGLFKPFVES YQIICKHLLS EEEDHFSEEQ YLAAVRKFTS QLLDQGTSQC YDVLSSDVQK NALAACVRLG VVEKKKINNN CIFNVNEPAT TKLEEMLGCK TPIGKPATAK L (331-680 aa encoded by BC000450)

Target Information

GNPAT is a key enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in GNPAT are associated with rhizomelic chondrodysplasia punctata type 2, which is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.

The Journal of clinical investigation

Park H,He A,Tan M,Johnson JM,Dean JM,Pietka TA,Chen Y,Zhang X,Hsu FF,Razani B,Funai K,Lodhi IJ

Fri Feb 01 00:00:00 EST 2019

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Cell host & microbe

Jean Beltran PM,Cook KC,Hashimoto Y,Galitzine C,Murray LA,Vitek O,Cristea IM

Wed Oct 10 00:00:00 EDT 2018

Differential activities of peroxisomes along the mouse intestinal epithelium.

Cell biochemistry and function

Morvay PL,Baes M,Van Veldhoven PP

Sat Apr 01 00:00:00 EDT 2017

C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.

Histochemistry and cell biology

Karnati S,Palaniswamy S,Alam MR,Oruqaj G,Stamme C,Baumgart-Vogt E

Tue Mar 01 00:00:00 EST 2016

Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.

Human mutation

Itzkovitz B,Jiralerspong S,Nimmo G,Loscalzo M,Horovitz DD,Snowden A,Moser A,Steinberg S,Braverman N

Sun Jan 01 00:00:00 EST 2012

Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3.

Human mutation

Itzkovitz B,Jiralerspong S,Nimmo G,Loscalzo M,Horovitz DD,Snowden A,Moser A,Steinberg S,Braverman N

Sun Jan 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: acyl-CoA:dihydroxyacetonephosphate acyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; DAP-AT; DHAP-AT; Dihydroxyacetone phosphate acyltransferase; Glycerone-phosphate O-acyltransferase

Gene Aliases: AU019525; D1Ertd819e; DAP-AT; DAPAT; DHAPAT; GNPAT; RCDP2

UniProt ID: (Human) O15228, (Mouse) P98192, (Rat) Q9ES71

Entrez Gene ID: (Human) 8443, (Mouse) 14712, (Rat) 84470

Molecular Function: acyltransferase