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Invitrogen
GOLPH3 Polyclonal Antibody
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FIGURE: 1 / 5
GOLPH3 Antibody (PA5-95558) in ICC/IF
Immunocytochemistry analysis of GOLPH3 using anti-GOLPH3 antibody (Product # PA5-95558) . GOLPH3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-GOLPH3 antibody (Product # PA5-95558) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was co... View More
Product Details
PA5-95558
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human GOLPH3 recombinant protein (Position: K56-K298).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human 293T whole cell, human MDA-MB-453 whole cell, human A431 whole cell, rat lung tissue, rat testis tissue, mouse lung tissue, mouse testis tissue. IHC: human intestinal cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GMx33
Gene Aliases: 4733401N08Rik; 5730410D03Rik; AW413496; Gmx33; GOLPH3; GOPP1; GPP34; MIDAS; Vps74
UniProt ID: (Human) Q9H4A6, (Mouse) Q9CRA5
Entrez Gene ID: (Human) 64083, (Mouse) 66629, (Rat) 78961
transport
Golgi organization
cell proliferation
glycoprotein biosynthetic process
protein secretion
gene expression
regulation of mitochondrion organization
protein transport
cell migration
lamellipodium assembly
positive regulation of TOR signaling
Golgi to plasma membrane protein transport
negative regulation of apoptotic process
protein retention in Golgi apparatus
Golgi vesicle budding
positive regulation of protein secretion
leukocyte tethering or rolling
cell adhesion molecule production
Golgi ribbon formation
asymmetric Golgi ribbon formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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