Proteintech

GOT1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

GOT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed L02 cells using 14886-1-AP (GOT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GOT1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14886-1-AP (GOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GOT1 Antibody in Immunoprecipitation (IP) — IP result of anti-GOT1 (IP:14886-1-AP, 4ug; Detection:14886-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

14886-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 8 publications 8 publications
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GOT1 Fusion Protein Ag6671 (1-413 aa encoded by BC000498) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.49 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAPPSVFAE VPQAQPVLVF KLTADFREDP DPRKVNLGVG AYRTDDCHPW VLPVVKKVEQ KIANDNSLNH EYLPILGLAE FRSCASRLAL GDDSPALKEK RVGGVQSLGG TGALRIGADF LARWYNGTNN KNTPVYVSSP TWENHNAVFS AAGFKDIRSY RYWDAEKRGL DLQGFLNDLE NAPEFSIVVL HACAHNPTGI DPTPEQWKQI ASVMKHRFLF PFFDSAYQGF ASGNLERDAW AIRYFVSEGF EFFCAQSFSK NFGLYNERVG NLTVVGKEPE SILQVLSQME KIVRITWSNP PAQGARIVAS TLSNPELFEE WTGNVKTMAD RILTMRSELR ARLEALKTPG TWNHITDQIG MFSFTGLNPK QVEYLVNEKH IYLLPSGRIN VSGLTTKNLD YVATSIHEAV TKIQ (1-413 aa encoded by BC000498)

Target Information

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Nature communications

Wang Y,Bai C,Ruan Y,Liu M,Chu Q,Qiu L,Yang C,Li B

Mon Jan 14 00:00:00 EST 2019

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Molecular medicine reports

Liu M,Liu S,Tan W,Tang F,Long J,Li Z,Liang B,Chu C,Yang J

Fri Dec 01 00:00:00 EST 2017

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

PloS one

Flannigan KL,Ferraz JG,Wang R,Wallace JL

Mon Apr 14 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A

Gene Aliases: AI789014; Aspat; AST1; ASTQTL1; cAspAT; cCAT; Gaspat; GIG18; Got-1; GOT1

UniProt ID: (Human) P17174, (Rat) P13221, (Mouse) P05201

Entrez Gene ID: (Human) 2805, (Rat) 24401, (Mouse) 14718

Molecular Function: transaminase