Proteintech

GOT2 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

GOT2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using GOT2 antibody 14800-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GOT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skin cancer using 14800-1-AP (GOT2 antibody) at dilution of 1:100 (under 40x lens).

GOT2 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GOT2 Antibody in Flow Cytometry (Flow) — 1X10^6 HEK-293 cells were stained with 0.2ug GOT2 antibody (14800-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

14800-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GOT2 Fusion Protein Ag6514 (82-430 aa encoded by BC000525) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: KAEAQIAAK NLDKEYLPIG GLAEFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRI GASFLQRFFK FSRDVFLPKP TWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FTGAVEDISK IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTMVCKDADE AKRVESQLKI LIRPMYSNPP LNGARIAAAI LNTPDLRKQW LQEVKVMADR IIGMRTQLVS NLKKEGSTHN WQHITDQIGM FCFTGLKPEQ VERLIKEFSI YMTKDGRISV AGVTSSNVGY LAHAIHQATK (82-430 aa encoded by BC000525)

Target Information

GOT2 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cell research

Cai WF,Zhang C,Wu YQ,Zhuang G,Ye Z,Zhang CS,Lin SC

Wed Aug 01 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Nature communications

Feist M,Schwarzfischer P,Heinrich P,Sun X,Kemper J,von Bonin F,Perez-Rubio P,Taruttis F,Rehberg T,Dettmer K,Gronwald W,Reinders J,Engelmann JC,Dudek J,Klapper W,Trümper L,Spang R,Oefner PJ,Kube D

Tue Apr 17 00:00:00 EDT 2018

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Nature communications

Wang YQ,Wang HL,Xu J,Tan J,Fu LN,Wang JL,Zou TH,Sun DF,Gao QY,Chen YX,Fang JY

Wed Feb 07 00:00:00 EST 2018

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Journal of cellular and molecular medicine

Li W,Chen C,Zhao X,Ye H,Zhao Y,Fu Z,Pan W,Zheng S,Wei L,Nong T,Li Z,Chen R

Wed Nov 01 00:00:00 EDT 2017

Glutamine metabolism regulates autophagy-dependent mTORC1 reactivation during amino acid starvation.

Nature communications

Tan HWS,Sim AYL,Long YC

Thu Aug 24 00:00:00 EDT 2017

Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.

Oncotarget

Kitazawa S,Ebara S,Ando A,Baba Y,Satomi Y,Soga T,Hara T

Tue Apr 25 00:00:00 EDT 2017

Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

Journal of proteome research

Qin S,Zhou Y,Gray L,Kusebauch U,McEvoy L,Antoine DJ,Hampson L,Park KB,Campbell D,Caballero J,Glusman G,Yan X,Kim TK,Yuan Y,Wang K,Rowen L,Moritz RL,Omenn GS,Pirmohamed M,Hood L

Fri Oct 07 00:00:00 EDT 2016

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; aspartate transaminase 2; FABP-1; FABPpm; Fatty acid-binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase 2, mitochondrial (aspartate aminotransferase 2); glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; mAspAT; mitochondrial aspartate aminotransferase; plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A

Gene Aliases: AL022787; ASPATA; FABP-pm; Got-2; GOT2; KAT4; KATIV; KYAT4; Maat; mAspAT; mitAAT

UniProt ID: (Human) P00505, (Mouse) P05202, (Rat) P00507

Entrez Gene ID: (Human) 2806, (Mouse) 14719, (Rat) 25721

Molecular Function: transaminase