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Invitrogen
GPAM Polyclonal Antibody
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FIGURE: 1 / 1
GPAM Antibody (PA5-93092) in WB
Western blot analysis of GPAM in extracts of various cell lines. Samples were incubated with GPAM Polyclonal antibody (Product # PA5-93092) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-93092
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.66 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LNKRGLGGPT STPPNLISQE QLVRKAASLC YLLSNEGTIS LPCQTFYQVC HETVGKFIQY GILTVAEHDD QEDISPSLAE QQWDKKLPEP LSWRSDEEDE DSDFGEEQRD CYLKVSQSKE HQQFITFLQR LLGPLLEAYS SAAIFVHNFS GPVPEPEYLQ KLHKYLITRT ERNVAVYAES ATYCLVKNAV KMFKDIGVFK ETKQKRVSVL ELSSTFLPQC NRQKLLEYIL SFVVL; Positive Samples: Mouse heart, Mouse lung, Rat brain; Cellular Location: Mitochondrion outer membrane, Multi-pass membrane protein
Target Information
Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycerol 3-phosphate acyltransferase, mitochondrial; Glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT; GPAT-1; P90; RP11-426E5.2
Gene Aliases: GPAM; GPAT; GPAT1; KIAA1560
UniProt ID: (Human) Q86TA3, (Mouse) Q61586, (Rat) P97564
Entrez Gene ID: (Human) 57678, (Mouse) 14732, (Rat) 29653
fatty acid metabolic process
acyl-CoA metabolic process
phosphatidic acid biosynthetic process
response to glucose
response to fructose
positive regulation of triglyceride biosynthetic process
response to activity
CDP-diacylglycerol biosynthetic process
triglyceride biosynthetic process
response to nutrient levels
cellular response to insulin stimulus
positive regulation of multicellular organism growth
positive regulation of activated T cell proliferation
response to cadmium ion
regulation of cytokine secretion
defense response to virus
fatty acid homeostasis
phospholipid homeostasis
negative regulation of activation-induced cell death of T cells
interleukin-2 secretion
lipid metabolic process
triglyceride metabolic process
glycerophospholipid metabolic process
metabolic process
phospholipid biosynthetic process
cellular lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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