Search
Invitrogen
GPD1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
GPD1 Antibody (PA5-96405) in WB
Western blot analysis of GPD1 in extracts of various cell lines. Samples were incubated with GPD1 Polyclonal antibody (Product # PA5-96405) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-96405
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GPD1 (NP_0052672)
Conjugate
Form
Liquid
Concentration
0.29 mg/mL
Amount
29 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM; Positive Samples: Mouse kidney, Mouse skeletal muscle, Rat liver; Cellular Location: Cytoplasm
Target Information
This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; glycerol 3-phosphate dehydrogenase; glycerol phosphate dehydrogenase 1, cytoplasmic adult; glycerol-3-phosphate dehydrogenase 1 (soluble); Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic; glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic; glycerolphosphate dehydrogenase 1, cytoplasmic adult; glycerophosphate dehydrogenase; GPD-C; GPDH-C; unnamed protein product
Gene Aliases: AI747587; Gdc-1; Gdc1; GPD-C; GPD1; Gpd3; GPDH; GPDH-C; HTGTI; Kiaa4010; mKIAA4010
UniProt ID: (Human) P21695, (Mouse) P13707, (Rat) O35077
Entrez Gene ID: (Human) 2819, (Mouse) 14555, (Rat) 60666
protein binding
oxidoreductase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
protein homodimerization activity
glycerol-3-phosphate dehydrogenase [NAD(P)+] activity
NAD binding
glycerol-3-phosphate dehydrogenase (NAD+) activity
glycerol-3-phosphate dehydrogenase [NAD+] activity
glycerol-3-phosphate dehydrogenase activity
dehydrogenase
carbohydrate metabolic process
glycerol-3-phosphate metabolic process
gluconeogenesis
glycerophosphate shuttle
positive regulation of glycolytic process
glycerol-3-phosphate catabolic process
cellular response to cAMP
cellular response to tumor necrosis factor
NADH oxidation
NADH metabolic process
glycerolipid metabolic process
oxidation-reduction process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support