Invitrogen

GPD1L Polyclonal Antibody

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View all (5) GPD1L antibodies

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GPD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis in formalin-fixed, paraffin-embedded mouse heart tissue using a GPD1L polyclonal antibody (Product # PA5-24216), followed by HRP-conjugated secondary antibody and DAB staining.

GPD1L Antibody in Western Blot (WB) — Western blot analysis using a GPD1L polyclonal antibody (Product # PA5-24216) in mouse heart tissue lysates (35 µg per lane).

GPD1L Antibody in Western Blot (WB) — Western blot analysis using a GPD1L polyclonal antibody (Product # PA5-24216) in MCF-7 cell lysates (35 µg per lane).

GPD1L Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of MCF-7 cells using a GPD1L polyclonal antibody (Product # PA5-24216) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

GPD1L Antibody in Western Blot (WB) — Fig. 4 210M induces glycolysis and lactate export of primary human astrocytes. A miRPathDB was used to predict pathways targeted by miR-210. The pathways with the most hits are listed in descending order, and the p -value of each pathway is included inside the respective bar. B Oxygen Consumption Rate (OCR) was measured in primary human astrocytes 48 h after transfection with 210S or 210M. The basal oxygen consumption (before oligomycin) and maximal respiratory capacity (between FCCP and Rotenone/Antimycin A) are presented in the histogram. n = 3 with 10 technical replicates for OCR experiments. C Extracellular Acidification Rate (ECAR) was measured in primary human astrocytes 48 h after transfection with 210S or 210M. The basal (before glucose), normal glycolysis (between glucose and oligomycin) and glycolytic capacity (between oligomycin and 2-DG addition) are presented in the histogram. n = 4 with 20 technical replicates for ECAR experiments. D RT-qPCR assessment of MCT4 in 210M-transfected cells relative to 210S-transfected cells 48 h after transfection. Mean +- SEM of 8 donors. E RT-qPCR assessment of GPD1L in 210M-transfected cells relative to 210S-transfected cells 48 h after transfection. Mean +- SEM of 8 donors. F Immunoblotted bands of GPD1L and Beta-tubulin (Beta-Tub) proteins and their quantification in 210M-transfected relative to 210S-transfected cells 48 h after transfection. Mean +- SEM of 6 donors. G Primary human astrocytes were transfected with 210S or 210M

Product Details

PA5-24216

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Flow Cytometry (Flow)

1:10-1:50

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 44-73 amino acids from the N-terminal region of human GPD1L

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2541716

Target Information

The protein encoded by this gene catalyzes the conversion of sn-glycerol 3-phosphate to glycerone phosphate. The encoded protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A). Defects in this gene are a cause of Brugada syndrome type 2 (BRS2) as well as sudden infant death syndrome (SIDS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes.

Journal of neuroinflammation

Kieran NW,Suresh R,Dorion MF,MacDonald A,Blain M,Wen D,Fuh SC,Ryan F,Diaz RJ,Stratton JA,Ludwin SK,Sonnen JA,Antel J,Healy LM

Published figure using GPD1L polyclonal antibody (Product # PA5-24216) in Western Blot

Thu Jan 06 00:00:00 EST 2022

Bioinformatics

Protein Aliases: Glycerol-3-phosphate dehydrogenase 1-like protein; GPD1-L

Gene Aliases: 2210409H23Rik; D9Ertd660e; GPD1-L; GPD1L; KIAA0089

UniProt ID: (Human) Q8N335, (Mouse) Q3ULJ0

Entrez Gene ID: (Human) 23171, (Mouse) 333433

Molecular Function: dehydrogenase

regulation of heart rate carbohydrate metabolic process phosphatidic acid biosynthetic process NADH metabolic process positive regulation of sodium ion transport triglyceride biosynthetic process NAD metabolic process negative regulation of peptidyl-serine phosphorylation glycerol-3-phosphate catabolic process oxidation-reduction process regulation of ventricular cardiac muscle cell membrane depolarization ventricular cardiac muscle cell action potential negative regulation of protein kinase C signaling positive regulation of protein localization to cell surface regulation of sodium ion transmembrane transporter activity glycerol-3-phosphate metabolic process

Process(es)

glycerol-3-phosphate dehydrogenase [NAD+] activity sodium channel regulator activity protein homodimerization activity ion channel binding NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor