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GPR109B Antibody (PA5-106832) in ICC/IF
Immunocytochemistry analysis of GPR109B in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GPR109B polyclonal antibody (Product # PA5-106832) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106832
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HCAR3(Accession P49019), corresponding to amino acid residues P302-P352.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2817661
Product Specific Information
Antibody detects endogenous levels of total GPR109.
Target Information
Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 109B; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; GTP-binding protein; hydroxy-carboxylic acid receptor 3; Hydroxycarboxylic acid receptor 3; Niacin receptor 2; Nicotinic acid receptor 2; putative chemokine receptor
Gene Aliases: GPR109B; HCA3; HCAR3; HM74; HM74B; NIACR2; Puma-g; PUMAG
UniProt ID: (Human) P49019
Entrez Gene ID: (Human) 8843
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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