Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
GPR120 Antibody (PA5-120658) in WB
Western blot analysis of GPR120 in extracts of various cell lines. Samples were incubated with GPR120 Polyclonal antibody (Product # PA5-120658) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120658
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 333-377 of human FFAR4 (NP_859529.2).
Conjugate
Form
Liquid
Concentration
1.7 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914230
Product Specific Information
Positive test controls include: HT-29, BxPC-3, RAW264.7, Mouse large intestine, Rat large intestine, Rat small intestine.^M
^M
Immunogen sequence: NPILYNMTLC RNEWKKIFCC FWFPEKGAIL TDTSVKRNDL SIISG
Target Information
An EST for GPR120 has been isolated from a human colon cancer library. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Free fatty acid receptor 4; G protein-coupled receptor 120; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; G-protein-coupled receptor GT01; GPCR120; Omega-3 fatty acid receptor 1
Gene Aliases: AI552415; BMIQ10; FFAR4; GPR120; GPR129; GT01; KPG_013; O3FAR1; PGR4
UniProt ID: (Human) Q5NUL3, (Mouse) Q7TMA4, (Rat) Q2AC31
Entrez Gene ID: (Human) 338557, (Mouse) 107221, (Rat) 294075
G-protein coupled receptor signaling pathway
regulation of glucose transport
cellular response to hormone stimulus
negative regulation of apoptotic process
fat cell differentiation
hormone secretion
negative regulation of cytokine secretion
negative regulation of inflammatory response
detection of chemical stimulus involved in sensory perception of taste
positive regulation of ERK1 and ERK2 cascade
response to peptide
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support