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Invitrogen
GPR18 Polyclonal Antibody
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FIGURE: 1 / 9
GPR18 Antibody (PA5-23218) in ICC/IF
Immunocytochemistry analysis of GPR18 in Ntera2 cells. Samples were incubated with GPR18 polyclonal antibody (Product # PA5-23218) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. Cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Product Details
PA5-23218
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Partial synthetic peptide made to an N-terminal portion of the human GPR18 protein (between amino acids 1-50).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR18 is an orphan G protein-coupled receptor found at high levels in the testis, small intestines and cells associated with the immune system including lymphocytes, thymus and spleen. GPR18 has been found to be a receptor for endogenous lipid neurotransmitters, several of which also bind to cannabinoid receptors. Some ligands described to be GPR18 agonists or partial agonists include N-Arachidonoylglycine, Abnormal cannabidiol, O-1602, AM-251, Cannabidiol, delta 9-Tetrahydrocannabinol, N-arachidonoyl ethanolamine and Arachidonylcyclopropylamide. Antagonists include the synthetic cannabidiol related compounds CID-85469571 and O-1918.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G-protein coupled receptor 18; gpr18; gpr18 {ECO:0000312|EMBL:AAI28783.1}; N-arachidonyl glycine receptor; NAGly receptor; RP11-461N23.8
Gene Aliases: GPCRW; GPR18
UniProt ID: (Human) Q14330, (Rat) A1A5S3, (Mouse) Q8K1Z6
Entrez Gene ID: (Human) 2841, (Rat) 679957, (Mouse) 110168
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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