Invitrogen

GPR87 Polyclonal Antibody

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GPR87 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GPR87 was performed on human kidney tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR87 polyclonal antibody (Product # PA3-043) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.

GPR87 Antibody in Western Blot (WB) — Western blot analysis of GPR87 was performed by loading equal amounts of wheat germ lectin agarose bead enriched GPR receptor fractions from GPR84 or GPR87 transfected HEK293 lysates onto a 7.5% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane, blocked and probed with a GPR87 polyclonal antibody (Product # PA3-043) at a dilution of 1:5000, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody. Denatured GPR84 was detected at ~72kDa. Chemiluminescent detection was performed using ECL.

Product Details

PA3-043

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:3000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated COOH-terminal amino acids of 339-358 (RSLQSVRRSEVRIYYDYTDV) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2664663

Product Specific Information

IHC(P) analysis shows positive staining of GPR87 in human kidney tubules.

WB analysis shows GPR87 in glyco-protein enriched fractions from GPR84 or GPR87 overexpressed 293 cells. The GPR87 antiserum showed specific detection of GPR87 over other tested GPRs.

GPR87 exists in different glycosylated forms.

Target Information

G protein-coupled receptors play a role in cell communication. They are characterized by an extracellular N terminus, 7 transmembrane regions, and an intracellular C terminus. GPR87 has been reported to be expressed in human prostate and rat stomach and fetal tissues. ESTs have been isolated from B-cell/lung/testis, breast, heart, jaw, prostate, and testis libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.