Invitrogen

GPS2 Polyclonal Antibody

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GPS2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of GPS2 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a GPS2 polyclonal antibody (Product # PA5-30953). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.

GPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GPS2 in paraffin-embedded gastric carcinoma using a GPS2 polyclonal antibody (Product # PA5-30953) at a dilution of 1:500.

GPS2 Antibody in Western Blot (WB) — Western blot analysis of GPS2 using 30 µg of A) A431 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GPS2 polyclonal antibody (Product # PA5-30953) at a dilution of 1:500.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:500

Immunofluorescence (IF)

1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1 and 253 of Human GPS2

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2548427

Product Specific Information

PA5-30953 targets GPS2 in IHC (P) and WB applications and shows reactivity with Human samples.

The PA5-30953 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 253 of Human GPS2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.