|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 153 and 375 of Human GPT|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29600 targets GPT in WB applications and shows reactivity with Human and mouse samples.
The PA5-29600 immunogen is recombinant fragment corresponding to a region within amino acids 153 and 375 of Human GPT.
Glutamate-pyruvate transaminase (EC 220.127.116.11), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.
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