|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 126 and 190 of Human GPX2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
PA5-27150 targets GPX2 in IHC (P) and WB applications and shows reactivity with human and mouse samples. Predicted to react with dog, monkey, pig, rat, and bovine based on 100% sequence homology.
The PA5-27150 immunogen is synthetic peptide corresponding to a region within amino acids 126 and 190 of Human GPX2.
This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Clinicopathological and prognostic significance of GPX2 protein expression in esophageal squamous cell carcinoma.
PA5-27150 was used in immunohistochemistry - paraffin section and western blot assess the use of GPX2 as a biomarker for esophageal squamous cell carcinoma
|Lei Z,Tian D,Zhang C,Zhao S,Su M||BMC cancer (16:null)||2016|