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Bethyl Laboratories
GR Polyclonal Antibody
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FIGURE: 1 / 1
GR Antibody (A303-388A) in WB
Detection of mouse GR by western blot and immunoprecipitation. Samples: Whole cell lysate from NIH 3T3 (M; 50 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-GR antibody (Product # A303-388A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. GR was also immunoprecipitated by rabbit anti-GR antibody A303-389A, which recognizes a disparate epitope. Detection: Chemiluminescence with exposure times of 60 seconds (A) and 10 seconds (B).
Product Details
A303-388A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 and 50 of mouse Glucocorticoid Receptor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GR; Grl; Grl-1; Grl1; Nr3c1
UniProt ID: (Mouse) P06537
Entrez Gene ID: (Mouse) 14815
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II regulatory region DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
glucocorticoid receptor activity
steroid binding
protein binding
transcription factor binding
zinc ion binding
lipid binding
Hsp70 protein binding
receptor tyrosine kinase binding
heat shock protein binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
hormone binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
steroid hormone binding
regulation of gluconeogenesis
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
glucocorticoid metabolic process
regulation of glucose metabolic process
positive regulation of glutamate secretion
chromatin modification
adrenal gland development
negative regulation of synaptic plasticity
regulation of glucocorticoid biosynthetic process
regulation of cell proliferation
maternal behavior
glucocorticoid receptor signaling pathway
negative regulation of vascular permeability
steroid hormone mediated signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
response to arsenic-containing substance
chromatin-mediated maintenance of transcription
mammary gland duct morphogenesis
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
cellular response to steroid hormone stimulus
cellular response to dexamethasone stimulus
negative regulation of glucocorticoid mediated signaling pathway
positive regulation of glucocorticoid receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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