Western blot analysis of GRAF in human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using a GRAF polyclonal antibody (Product # PA1-31264) at a dilution of 0.3µg/ml following incubation for 1 hour and detected using chemiluminescence.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide KTGLIPENYVEFL.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31264 detects GRAF from human, mouse, rat, dog, and cow samples.
PA1-31264 has been successfully used in Western blot applications.
The PA1-31264 immunogen is a synthetic peptide: KTGLIPENYVEFL.
Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.