Western blot analysis was performed on membrane enriched extracts of HCT-116 (Lane 1) and HT-29 (Lane 2). The blot was probed with Anti-GRASP55 Rabbit Polyclonal Antibody (Product # PA1-076, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/ml, 1:2500 dilution). A 55 kDa band corresponding to GRASP55 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues P(419) A S K V P T T V E D R V S D(433) of rat GRASP55 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-076 detects GRASP55 from mouse and rat samples.
PA1-076 has been successfully used in Western blot applications. By Western blot this antibody detects a ~55 kDa band representing the GRASP55 protein.
The PA1-076 immunogen is a synthetic peptide corresponding to residues P(419) A S K V P T T V E D R V S D(433) of rat GRASP55 protein. This peptide is available for neutralization controls as PEP-295.
GRASP55 is a PDZ containing protein involved in the stacking of golgi cisternea in a cell-free system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.