|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Residues 1-217 of GRB2.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25717 detects GRB2 from human, mouse, and rat samples.
PA1-25717 has been successfully used in Western blot procedures.
The PA1-25717 immunogen is residues 1-217 of GRB2.
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2 - Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signalling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.