Invitrogen

GRHL2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

2 References

View all (7) GRHL2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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GRHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human brain using an anti-GRHL2 polyclonal antibody (Product # PA5-41639). Primary antibody dilution: 5 µg/mL.

GRHL2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of using an anti-GRHL2 polyclonal antibody (Product # PA5-41639).

GRHL2 Antibody in Western Blot (WB) — Western blot analysis of human 721_B cell lysate using an anti-GRHL2 polyclonal antibody (Product # PA5-41639).

GRHL2 Antibody — GRHL2 promotes NSCLC cell growth and colony formation. H1299 cells were transfected with indicated siRNAs or not transfected. A. Western blot of GRHL2 to check the knockdown efficiency of siRNAs in the indicated cell lines; ß-actin was used as a loading control. B. Growth in low serum. H1299 cells werecultured in 1640 supplemented with 1% serum (low serum) or 10% serum (normal serum). After the indicated time, cells were trypsinized and counted. Data represent the average of three independent experiments. C. Colony formation assay. After the indicated times, H1299 were fixed and stained with crystal violet. Representative Wells were photographed and shown. {KD}

GRHL2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Published figure using GRHL2 polyclonal antibody (Product # PA5-41639) in Western Blot and Immunohistochemistry

Product Details

PA5-41639

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the middle region of human GRHL2
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607284

Product Specific Information

Peptide sequence: VEKIAKLYKK SKKGILVNMD DNIIEHYSNE DTFILNMESM VEGFKVTLME

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Target Information

The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.

American journal of translational research

Pan X,Zhang R,Xie C,Gan M,Yao S,Yao Y,Jin J,Han T,Huang Y,Gong Y,Wang J,Yu B

Published figure using GRHL2 polyclonal antibody (Product # PA5-41639) in Immunohistochemistry

Wed Sep 30 00:00:00 EDT 2020

GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.

American journal of translational research

Pan X,Zhang R,Xie C,Gan M,Yao S,Yao Y,Jin J,Han T,Huang Y,Gong Y,Wang J,Yu B

Published figure using GRHL2 polyclonal antibody (Product # PA5-41639) in Immunohistochemistry

Wed Sep 30 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Brother of mammalian grainyhead; grainyhead-like 2; Grainyhead-like protein 2 homolog; grainyhead-like transcription factor 2; Transcription factor CP2-like 3

Gene Aliases: BOM; DFNA28; ECTDS; GRHL2; TFCP2L3

UniProt ID: (Human) Q6ISB3

Entrez Gene ID: (Human) 79977

Molecular Function: DNA-binding transcription factor