Invitrogen

GRHL2 Polyclonal Antibody

GRHL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GRHL2 Antibody (PA5-41639) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human brain using an anti-GRHL2 polyclonal antibody (Product # PA5-41639). Primary antibody dilution: 5 µg/mL.

GRHL2 Antibody in Immunohistochemistry (IHC)

GRHL2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

GRHL2 Antibody — GRHL2 promotes NSCLC cell growth and colony formation. H1299 cells were transfected with indicated siRNAs or not transfected. A. Western blot of GRHL2 to check the knockdown efficiency of siRNAs in the indicated cell lines; ß-actin was used as a loading control. B. Growth in low serum. H1299 cells werecultured in 1640 supplemented with 1% serum (low serum) or 10% serum (normal serum). After the indicated time, cells were trypsinized and counted. Data represent the average of three independent experiments. C. Colony formation assay. After the indicated times, H1299 were fixed and stained with crystal violet. Representative Wells were photographed and shown. {KD}

Product Details

PA5-41639

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human GRHL2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607284

Product Specific Information

Peptide sequence: VEKIAKLYKK SKKGILVNMD DNIIEHYSNE DTFILNMESM VEGFKVTLME

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Target Information

The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.

American journal of translational research

Pan X,Zhang R,Xie C,Gan M,Yao S,Yao Y,Jin J,Han T,Huang Y,Gong Y,Wang J,Yu B

Published figure using GRHL2 polyclonal antibody (Product # PA5-41639) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.

American journal of translational research

Pan X,Zhang R,Xie C,Gan M,Yao S,Yao Y,Jin J,Han T,Huang Y,Gong Y,Wang J,Yu B

Published figure using GRHL2 polyclonal antibody (Product # PA5-41639) in Western Blot

Wed Sep 30 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Brother of mammalian grainyhead; grainyhead-like 2; Grainyhead-like protein 2 homolog; grainyhead-like transcription factor 2; Transcription factor CP2-like 3

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Gene Aliases: BOM; DFNA28; ECTDS; GRHL2; TFCP2L3

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UniProt ID: (Human) Q6ISB3

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Entrez Gene ID: (Human) 79977

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Function(s)

intronic transcription regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding chromatin DNA binding sequence-specific DNA binding DNA-binding transcription factor

Process(es)

neural tube closure cardiac ventricle morphogenesis epithelial cell morphogenesis transcription from RNA polymerase II promoter cell adhesion brain development cell proliferation epidermis development neural tube development cell junction assembly multicellular organism growth embryonic digit morphogenesis camera-type eye development regulation of DNA methylation negative regulation of keratinocyte differentiation positive regulation of transcription from RNA polymerase II promoter embryonic cranial skeleton morphogenesis positive regulation of telomerase activity face development lung lobe morphogenesis lung epithelial cell differentiation epithelial cell morphogenesis involved in placental branching bicellular tight junction assembly epithelium migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRHL2 Polyclonal Antibody

GRHL2 Antibody (PA5-41639) in IHC (P)

Product Details

PA5-41639

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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