Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human GRK2. A BLAST analysis of the sequence shows 100% reactivity to human, and 90% to rat, mouse, and golden hamster.
G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adrenergic beta receptor kinase 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-AR kinase-1; Beta-ARK-1; EC 18.104.22.168; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2
Gene Aliases: Adrbk-1; ADRBK1; BARK; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK2
Molecular Function: non-receptor serine/threonine protein kinase