Proteintech

GRP75 Polyclonal Antibody

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GRP75 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

GRP75 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 14887-1-AP (GRP75 antibody) at dilution of 1:100 (under 40x lens).

GRP75 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GRP75 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug GRP75 antibody (14887-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

GRP75 Antibody in Immunoprecipitation (IP) — IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:2000) with mouse brain tissue lysate 3680ug.

Product Details

14887-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GRP75 Fusion Protein Ag6673 (331-679 aa encoded by BC000478)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody recognized the endogenous HSPA9 protein in NIH/3T3 cells.

Immunogen sequence: YLTMDSSGP KHLNMKLTRA QFEGIVTDLI RRTIAPCQKA MQDAEVSKSD IGEVILVGGM TRMPKVQQTV QDLFGRAPSK AVNPDEAVAI GAAIQGGVLA GDVTDVLLLD VTPLSLGIET LGGVFTKLIN RNTTIPTKKS QVFSTAADGQ TQVEIKVCQG EREMAGDNKL LGQFTLIGIP PAPRGVPQIE VTFDIDANGI VHVSAKDKGT GREQQIVIQS SGGLSKDDIE NMVKNAEKYA EEDRRKKERV EAVNMAEGII HDTETKMEEF KDQLPADECN KLKEEISKMR ELLARKDSET GENIRQAASS LQQASLKLFE MAYKKMASER EGSGSSGTGE QKEDQKEEKQ (331-679 aa encoded by BC000478)

Target Information

The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; Heat shock protein; heat shock protein 9A; heat shock protein cognate 74; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; mortalin, perinuclear; mortalin-2; mortalin2; MOT; mtHSP70; p66 MOT; p66-mortalin; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial

Gene Aliases: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; Mortalin; MOT; Mot-2; MOT2; mt-HSP70; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4

UniProt ID: (Human) P38646, (Mouse) P38647

Entrez Gene ID: (Human) 3313, (Rat) 291671, (Mouse) 15526

Function(s)

protein folding protein export from nucleus response to toxic substance cellular response to interleukin-1 erythrocyte differentiation negative regulation of apoptotic process negative regulation of erythrocyte differentiation

Process(es)

protein binding ATP binding fibroblast growth factor binding heat shock protein binding ubiquitin protein ligase binding poly(A) RNA binding unfolded protein binding enzyme binding nucleotide binding