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Invitrogen
GRP78 Polyclonal Antibody
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Product Details
PA5-24963
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Zebrafish
Published species
Human,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 261-289 amino acids from the central region of human HSPA5
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
200 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken, mouse and rat based on sequence homology.
Target Information
GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78's involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; chaperone; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose-regulated protein (grp78); glucose-regulated protein, 78kDa; GRP-78; heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein family A member 5; HSP70 family protein 5; HSPA 5; Immunoglobulin heavy chain-binding protein; unnamed protein product
Gene Aliases: BIP; cb865; fb60h09; fi36d04; GRP78; HEL-S-89n; HSPA5; wu:fb60h09; wu:fi36d04; zgc:55994; zgc:77606
UniProt ID: (Human) P11021
Entrez Gene ID: (Human) 3309, (Zebrafish) 378848
nucleotide binding
calcium ion binding
protein binding
ATP binding
hydrolase activity
ATPase activity
enzyme binding
protein domain specific binding
protein serine/threonine kinase inhibitor activity
heat shock protein binding
ubiquitin protein ligase binding
ribosome binding
protein binding involved in protein folding
cadherin binding
unfolded protein binding
chaperone binding
misfolded protein binding
ATP-dependent protein folding chaperone
response to hypoxia
response to yeast
response to unfolded protein
ER-associated ubiquitin-dependent protein catabolic process
endoplasmic reticulum unfolded protein response
cellular response to unfolded protein
protein refolding
chaperone mediated protein folding requiring cofactor
translational initiation
protein folding
ER overload response
cerebellum structural organization
cerebellar Purkinje cell layer development
substantia nigra development
positive regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
posttranslational protein targeting to membrane, translocation
negative regulation of protein complex assembly
positive regulation of protein ubiquitination
protein folding in endoplasmic reticulum
response to endoplasmic reticulum stress
maintenance of protein localization in endoplasmic reticulum
IRE1-mediated unfolded protein response
PERK-mediated unfolded protein response
ERAD pathway
cellular response to glucose starvation
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
proteolysis involved in cellular protein catabolic process
regulation of protein folding in endoplasmic reticulum
cellular response to interleukin-4
regulation of ATF6-mediated unfolded protein response
regulation of IRE1-mediated unfolded protein response
negative regulation of IRE1-mediated unfolded protein response
regulation of PERK-mediated unfolded protein response
negative regulation of PERK-mediated unfolded protein response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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