Proteintech

GRP94 Polyclonal Antibody

GRP94 Antibody in Immunocytochemistry (ICC/IF)

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GRP94 Antibody (14700-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

GRP94 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GRP94 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14700-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GRP94 Fusion Protein Ag6344 (455-803 aa encoded by BC066656)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody was generated against the C-terminal region of full-length HSP90b1.

Immunogen sequence: KLLKVIRKK LVRKTLDMIK KIADDKYNDT FWKEFGTNIK LGVIEDHSNR TRLAKLLRFQ SSHHPTDITS LDQYVERMKE KQDKIYFMAG SSRKEAESSP FVERLLKKGY EVIYLTEPVD EYCIQALPEF DGKRFQNVAK EGVKFDESEK TKESREAVEK EFEPLLNWMK DKALKDKIEK AVVSQRLTES PCALVASQYG WSGNMERIMK AQAYQTGKDI STNYYASQKK TFEINPRHPL IRDMLRRIKE DEDDKTVLDL AVVLFETATL RSGYLLPDTK AYGDRIERML RLSLNIDPDA KVEEEPEEEP EETAEDTTED TEQDEDEEMD VGTDEEEETA KESTAEKDEL (455-803 aa encoded by BC066656)

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Neuroscience

Ko AR,Kim JY,Hyun HW,Kim JE

Thu Oct 29 00:00:00 UTC 2015

Endoplasmic reticulum (ER) stress protein responses in relation to spatio-temporal dynamics of astroglial responses to status epilepticus in rats.

Neuroscience

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Reduction in heat shock protein 90 correlates to neuronal vulnerability in the rat piriform cortex following status epilepticus.

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Thu Aug 14 00:00:00 UTC 2014

Reduction in heat shock protein 90 correlates to neuronal vulnerability in the rat piriform cortex following status epilepticus.

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Kim YJ,Kim JY,Ko AR,Kang TC

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Reduction in heat shock protein 90 correlates to neuronal vulnerability in the rat piriform cortex following status epilepticus.

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Bioinformatics

Protein Aliases: 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; ERp99; gp96 homolog; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family C member 4; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Polymorphic tumor rejection antigen 1; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96

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Gene Aliases: ECGP; endoplasmin; ERp99; GP96; GRP94; HEL-S-125m; HEL35; HSP90B1; HSPC4; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Mouse) P08113, (Rat) Q66HD0

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Entrez Gene ID: (Human) 7184, (Mouse) 22027, (Rat) 362862

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Function(s)

RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding nucleotide binding Hsp90 family chaperone

Process(es)

response to hypoxia toll-like receptor signaling pathway receptor-mediated endocytosis protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ATF6-mediated unfolded protein response negative regulation of apoptotic process regulation of phosphoprotein phosphatase activity sequestering of calcium ion cellular response to ATP protein folding response to stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRP94 Polyclonal Antibody

GRP94 Antibody (14700-1-AP) in ICC/IF

Product Details

14700-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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