|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from c-terminus of human GSK-3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Glycogen Synthase Kinase-3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3alpha and GSK-3beta. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.