Proteintech

GSR Polyclonal Antibody

GSR Antibody in Immunocytochemistry (ICC/IF)

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GSR Antibody (18257-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells, using GSR antibody 18257-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

GSR Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18257-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GSR Fusion Protein Ag13080 (274-522 aa encoded by BC069244)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to GSR.

Immunogen sequence: ISTNCTEEL ENAGVEVLKF SQVKEVKKTL SGLEVSMVTA VPGRLPVMTM IPDVDCLLWA IGRVPNTKDL SLNKLGIQTD DKGHIIVDEF QNTNVKGIYA VGDVCGKALL TPVAIAAGRK LAHRLFEYKE DSKLDYNNIP TVVFSHPPIG TVGLTEDEAI HKYGIENVKT YSTSFTPMYH AVTKRKTKCV MKMVCANKEE KVVGIHMQGL GCDEMLQGFA VAVKMGATKA DFDNTVAIHP TSSEELVTLR (274-522 aa encoded by BC069244)

Target Information

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis.

Journal of biomedical science

Hung YW,Ouyang C,Ping X,Qi Y,Wang YC,Kung HJ,Ann DK

Mon May 22 00:00:00 UTC 2023

A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites.

EBioMedicine

Wang Y,Tang B,Li H,Zheng J,Zhang C,Yang Z,Tan X,Luo P,Ma L,Wang Y,Long L,Chen Z,Xiao Z,Ma L,Zhou J,Wang Y,Shi C

Sat Apr 01 00:00:00 UTC 2023

Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.

Redox biology

Gallego-Selles A,Galvan-Alvarez V,Martinez-Canton M,Garcia-Gonzalez E,Morales-Alamo D,Santana A,Gonzalez-Henriquez JJ,Dorado C,Calbet JAL,Martin-Rincon M

Thu Sep 01 00:00:00 UTC 2022

Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.

Pharmacological research

Chang MT,Tsai LC,Nakagawa-Goto K,Lee KH,Shyur LF

Fri Apr 01 00:00:00 UTC 2022

Glucose-6-phosphate dehydrogenase exerts antistress effects independently of its enzymatic activity.

The Journal of biological chemistry

Jin X,Li X,Li L,Zhong B,Hong Y,Niu J,Li B

Thu Dec 01 00:00:00 UTC 2022

Anti-Inflammatory Effect of Dimethyl Fumarate Associates with the Inhibition of Thioredoxin Reductase 1 in RAW 264.7 Cells.

Molecules (Basel, Switzerland)

Yang R,Sun S,Guo Y,Meng Y,Liu H,Shi M,Guan S,Xu J

Fri Dec 23 00:00:00 UTC 2022

GSK 650394 Inhibits Osteoclasts Differentiation and Prevents Bone Loss via Promoting the Activities of Antioxidant Enzymes In Vitro and In Vivo.

Oxidative medicine and cellular longevity

Jin LY,Huo SC,Guo C,Liu HY,Xu S,Li XF

Wed Sep 28 00:00:00 UTC 2022

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Journal of food biochemistry

Wang Z,Cai L,Li H,Liang M,Zhang Y,Wu Q,Yang L

Fri May 01 00:00:00 UTC 2020

Desflurane Preconditioning Protects Against Renal Ischemia-Reperfusion Injury and Inhibits Inflammation and Oxidative Stress in Rats Through Regulating the Nrf2-Keap1-ARE Signaling Pathway.

Drug design, development and therapy

Zheng Y,Lu H,Huang H

Mon Jan 18 00:00:00 UTC 2021

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Journal of the science of food and agriculture

Wang Z,Liang M,Li H,Cai L,He H,Wu Q,Yang L

Thu Aug 15 00:00:00 UTC 2019

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Free radical biology & medicine

Blecha J,Novais SM,Rohlenova K,Novotna E,Lettlova S,Schmitt S,Zischka H,Neuzil J,Rohlena J

Wed Nov 01 00:00:00 UTC 2017

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Free radical biology & medicine

Blecha J,Novais SM,Rohlenova K,Novotna E,Lettlova S,Schmitt S,Zischka H,Neuzil J,Rohlena J

Wed Nov 01 00:00:00 UTC 2017

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Free radical biology & medicine

Dong K,Wu M,Liu X,Huang Y,Zhang D,Wang Y,Yan LJ,Shi D

Thu Dec 01 00:00:00 UTC 2016

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Free radical biology & medicine

Dong K,Wu M,Liu X,Huang Y,Zhang D,Wang Y,Yan LJ,Shi D

Thu Dec 01 00:00:00 UTC 2016

GSH-dependent antioxidant defense contributes to the acclimation of colon cancer cells to acidic microenvironment.

Cell cycle (Georgetown, Tex.)

Zhao M,Liu Q,Gong Y,Xu X,Zhang C,Liu X,Zhang C,Guo H,Zhang X,Gong Y,Shao C

Fri Jan 06 00:00:00 UTC 2017

The Essential Role of H19 Contributing to Cisplatin Resistance by Regulating Glutathione Metabolism in High-Grade Serous Ovarian Cancer.

Scientific reports

Zheng ZG,Xu H,Suo SS,Xu XL,Ni MW,Gu LH,Chen W,Wang LY,Zhao Y,Tian B,Hua YJ

Thu May 19 00:00:00 UTC 2016

The Essential Role of H19 Contributing to Cisplatin Resistance by Regulating Glutathione Metabolism in High-Grade Serous Ovarian Cancer.

Scientific reports

Zheng ZG,Xu H,Suo SS,Xu XL,Ni MW,Gu LH,Chen W,Wang LY,Zhao Y,Tian B,Hua YJ

Thu May 19 00:00:00 UTC 2016

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Cell reports

Wu H,Xiao Y,Zhang S,Ji S,Wei L,Fan F,Geng J,Tian J,Sun X,Qin F,Jin C,Lin J,Yin ZY,Zhang T,Luo L,Li Y,Song S,Lin SC,Deng X,Camargo F,Avruch J,Chen L,Zhou D

Thu May 30 00:00:00 UTC 2013

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Cell reports

Wu H,Xiao Y,Zhang S,Ji S,Wei L,Fan F,Geng J,Tian J,Sun X,Qin F,Jin C,Lin J,Yin ZY,Zhang T,Luo L,Li Y,Song S,Lin SC,Deng X,Camargo F,Avruch J,Chen L,Zhou D

Thu May 30 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: epididymis luminal protein 75; epididymis secretory sperm binding protein Li 122m; glutathione reductase; glutathione reductase 1; Glutathione reductase, mitochondrial; glutathione S-reductase; GR; GRase

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Gene Aliases: AI325518; D8Ertd238e; GLUR; Gr-1; Gr1; GRD1; GSR; HEL-75; HEL-S-122m

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UniProt ID: (Human) P00390, (Mouse) P47791

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Entrez Gene ID: (Human) 2936, (Mouse) 14782, (Rat) 116686

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Function(s)

glutathione-disulfide reductase activity electron carrier activity flavin adenine dinucleotide binding NADP binding oxidoreductase activity oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor protein homodimerization activity glutathione binding oxidoreductase reductase metabolite interconversion enzyme

Process(es)

response to reactive oxygen species glutathione metabolic process nucleobase-containing small molecule interconversion cell redox homeostasis oxidation-reduction process cellular oxidant detoxification spermatogenesis response to vitamin A response to nicotine response to ethanol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GSR Polyclonal Antibody

GSR Antibody (18257-1-AP) in ICC/IF

Product Details

18257-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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