Proteintech

GSS Polyclonal Antibody

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GSS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed MDCK cells using 15712-1-AP (GSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 15712-1-AP ( GSS Antibody) at dilution of 1:200 (under 40x lens).

GSS Antibody in Western Blot (WB) — CHO cells were subjected to SDS PAGE followed by western blot with 15712-1-AP (GSS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

15712-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GSS Fusion Protein Ag8338 (125-474 aa encoded by BC007927)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: RSDYMFQRS ADGSPALKQI EINTISASFG GLASRTPAVH RHVLSVLSKT KEAGKILSNN PSKGLALGIA KAWELYGSPN ALVLLIAQEK ERNIFDQRAI ENELLARNIH VIRRTFEDIS EKGSLDQDRR LFVDGQEIAV VYFRDGYMPR QYSLQNWEAR LLLERSHAAK CPDIATQLAG TKKVQQELSR PGMLEMLLPG QPEAVARLRA TFAGLYSLDV GEEGDQAIAE ALAAPSRFVL KPQREGGGNN LYGEEMVQAL KQLKDSEERA SYILMEKIEP EPFENCLLRP GSPARVVQCI SELGIFGVYV RQEKTLVMNK HVGHLLRTKA IEHADGGVAA GVAVLDNPYP V (125-474 aa encoded by BC007927)

Target Information

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

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γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.

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Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

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Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

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Bioinformatics

Protein Aliases: Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB; MGC14098; OTTHUMP00000030711

Gene Aliases: AI314904; GS-A/GS-B; GSH-S; GSHS; GSS; HEL-S-64p; HEL-S-88n

UniProt ID: (Human) P48637, (Rat) P46413, (Mouse) P51855

Entrez Gene ID: (Human) 2937, (Pig) 100152660, (Dog) 442962, (Rat) 25458, (Mouse) 14854, (Chinese hamster) 100753200

Molecular Function: ligase metabolite interconversion enzyme

Function(s)

cellular amino acid metabolic process glutathione biosynthetic process response to oxidative stress nervous system development aging response to xenobiotic stimulus response to nutrient levels response to tumor necrosis factor response to amino acid response to cadmium ion

Process(es)

magnesium ion binding glutathione synthase activity protein binding ATP binding glycine binding protein homodimerization activity glutathione binding peptide binding nucleotide binding ligase activity metal ion binding