Proteintech

GSS Polyclonal Antibody

View all (25) GSS antibodies

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Questions & Answers

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GSS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed MDCK cells using 15712-1-AP (GSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 15712-1-AP ( GSS Antibody) at dilution of 1:200 (under 40x lens).

GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 15712-1-AP ( GSS Antibody) at dilution of 1:200 (under 10x lens).

GSS Antibody in Western Blot (WB) — CHO cells were subjected to SDS PAGE followed by western blot with 15712-1-AP (GSS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

GSS Antibody in Western Blot (WB) — CHO cells were subjected to SDS PAGE followed by western blot with 15712-1-AP (GSS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

15712-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSS Fusion Protein Ag8338 (125-474 aa encoded by BC007927) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RSDYMFQRS ADGSPALKQI EINTISASFG GLASRTPAVH RHVLSVLSKT KEAGKILSNN PSKGLALGIA KAWELYGSPN ALVLLIAQEK ERNIFDQRAI ENELLARNIH VIRRTFEDIS EKGSLDQDRR LFVDGQEIAV VYFRDGYMPR QYSLQNWEAR LLLERSHAAK CPDIATQLAG TKKVQQELSR PGMLEMLLPG QPEAVARLRA TFAGLYSLDV GEEGDQAIAE ALAAPSRFVL KPQREGGGNN LYGEEMVQAL KQLKDSEERA SYILMEKIEP EPFENCLLRP GSPARVVQCI SELGIFGVYV RQEKTLVMNK HVGHLLRTKA IEHADGGVAA GVAVLDNPYP V (125-474 aa encoded by BC007927)

Target Information

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Journal of molecular medicine (Berlin, Germany)

Peng L,Zhao Y,Li Y,Zhou Y,Li L,Lei S,Yu S,Zhao Y

Fri Feb 01 00:00:00 EST 2019

γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.

Redox biology

Yang Y,Li L,Hang Q,Fang Y,Dong X,Cao P,Yin Z,Luo L

Tue Jan 01 00:00:00 EST 2019

Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Maclean KN,Jiang H,Aivazidis S,Kim E,Shearn CT,Harris PS,Petersen DR,Allen RH,Stabler SP,Roede JR

Thu Mar 01 00:00:00 EST 2018

Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders.

International journal of molecular medicine

Wang H,Xu YS,Wang ML,Cheng C,Bian R,Yuan H,Wang Y,Guo T,Zhu LL,Zhou H

Sat Apr 01 00:00:00 EDT 2017

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Jiang G,Zhang G,An T,He Z,Kang L,Yang X,Gu Y,Zhang D,Wang Y,Gao S

Mon Feb 06 00:00:00 EST 2017

Potent effects of dioscin against obesity in mice.

Scientific reports

Liu M,Xu L,Yin L,Qi Y,Xu Y,Han X,Zhao Y,Sun H,Yao J,Lin Y,Liu K,Peng J

Thu Jan 22 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB; MGC14098; OTTHUMP00000030711

Gene Aliases: AI314904; GS-A/GS-B; GSH-S; GSHS; GSS; HEL-S-64p; HEL-S-88n

UniProt ID: (Human) P48637, (Rat) P46413, (Mouse) P51855

Entrez Gene ID: (Human) 2937, (Rat) 25458, (Mouse) 14854

Molecular Function: ligase

cellular amino acid metabolic process glutathione biosynthetic process response to oxidative stress nervous system development aging response to xenobiotic stimulus response to nutrient levels response to tumor necrosis factor response to amino acid response to cadmium ion

Process(es)

magnesium ion binding glutathione synthase activity protein binding ATP binding glycine binding protein homodimerization activity glutathione binding peptide binding nucleotide binding ligase activity metal ion binding