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Invitrogen
GST Tag Polyclonal Antibody
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GST Tag Antibody (A-5800) in WB
Western blot was performed using Anti-GST Tag Polyclonal Antibody (Product # A-5800) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-GST, 50 µg (Lane 3), H3-GST, 25 µg (Lane 4), H3-GST, 12.5 µg (Lane 5), H3-GST, 6.25 µg (Lane 6), GST-p65, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB2300... View More
Product Details
A-5800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
C. elegans,
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST isolated from Schistosoma japonicum
Conjugate
Form
Liquid
Concentration
3 mg/mL
Purification
IgG fraction
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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