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          • Primary Antibodies ›
          • GST Antibodies

          Invitrogen

          GST Tag Polyclonal Antibody

          Advanced Verification
          21 Published Figures
          86 References
          View all (101) GST antibodies

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          Cite GST Tag Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (21)
          • Advanced Verification (1)
          GST Tag Antibody in Western Blot (WB)
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          GST Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 24

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          GST Tag Antibody (A-5800) in WB

          Western blot was performed using Anti-GST Tag Polyclonal Antibody (Product # A-5800) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), H3-GST, 50 µg (Lane 3), H3-GST, 25 µg (Lane 4), H3-GST, 12.5 µg (Lane 5), H3-GST, 6.25 µg (Lane 6), GST-p65, 50 µg (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB2300... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in ELISA (ELISA)
          GST Tag Antibody in ELISA (ELISA)
          GST Tag Antibody

          Product Details

          A-5800

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 39 publications 39 publications

          Immunocytochemistry (ICC/IF)

          -
          View 13 publications 13 publications

          ELISA (ELISA)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 5 publications 5 publications

          ChIP assay (ChIP)

          -
          View 2 publications 2 publications

          Functional Assay (Functional)

          -
          View 1 publication 1 publication

          Luminex (Luminex)

          -
          View 1 publication 1 publication

          Inhibition Assays (Inhibition)

          -
          View 1 publication 1 publication

          Immunoelectrophoresis (IE)

          -
          View 1 publication 1 publication

          Affinity Purification (AP)

          -
          View 1 publication 1 publication

          Mass Spectrometry (MS)

          -
          View 6 publications 6 publications

          Miscellaneous PubMed (Misc)

          -
          View 13 publications 13 publications
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          GST isolated from Schistosoma japonicum

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          3 mg/mL

          Amount

          1.5 mg

          Purification

          IgG fraction

          Storage buffer

          PBS, pH 7.5

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536195

          Target Information

          GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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