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GSTO1 Polyclonal Antibody

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GSTO1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using GSTO1 antibody 15124-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

GSTO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 15124-1-AP (GSTO1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

GSTO1 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 15124-1-AP (GSTO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

GSTO1 Antibody in Immunoprecipitation (IP) — IP result of anti-GSTO1 (IP:15124-1-AP, 4ug; Detection:15124-1-AP 1:500) with Jurkat cells lysate 4000ug.

Product Details

15124-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSTO1 Fusion Protein Ag7199 (1-241 aa encoded by BC000127) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa).

Immunogen sequence: MSGESARSL GKGSAPPGPV PEGSIRIYSM RFCPFAERTR LVLKAKGIRH EVININLKNK PEWFFKKNPF GLVPVLENSQ GQLIYESAIT CEYLDEAYPG KKLLPDDPYE KACQKMILEL FSKVPSLVGS FIRSQNKEDY AGLKEEFRKE FTKLEEVLTN KKTTFFGGNS ISMIDYLIWP WFERLEAMKL NECVDHTPKL KLWMAAMKED PTVSALLTSE KDWQGFLELY LQNSPEACDY GL (1-241 aa encoded by BC000127)

Target Information

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of NRF2 signaling and increased reactive oxygen species during embryogenesis in a rat model of retinoic acid-induced neural tube defects.

Neurotoxicology

Liu D,Xue J,Liu Y,Gu H,Wei X,Ma W,Luo W,Ma L,Jia S,Dong N,Huang J,Wang Y,Yuan Z

Sat Dec 01 00:00:00 EST 2018

Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.

Journal of proteomics

Zhang D,Sun X,Ren L,Yang C,Liu X,Zhang H,Jiang Y,Hu X

Thu Aug 30 00:00:00 EDT 2018

The interaction of arsenic and N-butyl-N-(4-hydroxybutyl)nitrosamine on urothelial carcinogenesis in mice.

PloS one

Dai YC,Wang SC,Haque MM,Lin WH,Lin LC,Chen CH,Liu YW

Thu Oct 19 00:00:00 EDT 2017

Selenophosphate synthetase 1 is an essential protein with roles in regulation of redox homoeostasis in mammals.

The Biochemical journal

Tobe R,Carlson BA,Huh JH,Castro NP,Xu XM,Tsuji PA,Lee SG,Bang J,Na JW,Kong YY,Beaglehole D,Southon E,Seifried H,Tessarollo L,Salomon DS,Schweizer U,Gladyshev VN,Hatfield DL,Lee BJ

Fri Jul 15 00:00:00 EDT 2016

Downregulation of glutathione S-transferase M1 protein in N-butyl-N-(4-hydroxybutyl)nitrosamine-induced mouse bladder carcinogenesis.

Toxicology and applied pharmacology

Chuang JJ,Dai YC,Lin YL,Chen YY,Lin WH,Chan HL,Liu YW

Mon Sep 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: epididymis secretory protein Li 21; glutathione S-transferase like; Glutathione S-transferase omega 1-1; Glutathione S-transferase omega-1; Glutathione transferase omega 1 (GSTO 1-1) (p28); Glutathione-dependent dehydroascorbate reductase; glutathione-S-transferase like; GSTO 1-1; GSTO-1; MMA(V) reductase; Monomethylarsonic acid reductase; p28; RP11-99N20.1; S-(Phenacyl)glutathione reductase; SPG-R

Gene Aliases: AA407097; AI194287; AU018802; GSTO 1-1; GSTO1; GSTTLP28; GSTX; Gtsttl; HEL-S-21; P28; SPG-R

UniProt ID: (Human) P78417, (Mouse) O09131

Entrez Gene ID: (Human) 9446, (Rat) 114846, (Mouse) 14873

Function(s)

metabolic process regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum L-ascorbic acid metabolic process L-ascorbic acid biosynthetic process xenobiotic catabolic process oxidation-reduction process negative regulation of ryanodine-sensitive calcium-release channel activity positive regulation of ryanodine-sensitive calcium-release channel activity cellular response to arsenic-containing substance glutathione metabolic process regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion positive regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion methylation cellular oxidant detoxification glutathione derivative biosynthetic process

Process(es)

glutathione transferase activity protein binding oxidoreductase activity glutathione dehydrogenase (ascorbate) activity methylarsonate reductase activity transferase activity

GSTO1 Polyclonal Antibody

Product Details

15124-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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