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Immunofluorescent analysis of GTF2I showing staining in the nucleus of A549 cells using a GTF2I polyclonal antibody (Product # PA5-35321) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat based on sequence homology.
GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BAP135; Bruton tyrosine kinase-associated protein 135; BTK-associated protein, 135kD; GTF2I; SRF-Phox1-interacting protein; WBSCR6; Williams-Beuren syndrome chromosome region 6
BAP135; BTKAP1; DIWS; GTF2I; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6