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Invitrogen
GTF3A Polyclonal Antibody
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FIGURE: 1 / 1
GTF3A Antibody (PA5-97049) in WB
Western blot analysis of GTF3A in 293F cells. Samples were incubated with GTF3A Polyclonal antibody (Product # PA5-97049) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-97049
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_0020882)
Conjugate
Form
Liquid
Concentration
0.78 mg/mL
Amount
78 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF; Positive Samples: SW480; Cellular Location: Nucleus
Target Information
The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TF3A; TFIIIA; Transcription factor IIIA
Gene Aliases: AP2; GTF3A; TFIIIA
UniProt ID: (Human) Q92664
Entrez Gene ID: (Human) 2971
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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