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Agrisera
GUS/Beta-glucuronidase Polyclonal Antibody
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FIGURE: 1 / 1
GUS/Beta-glucuronidase Antibody (AS16-3689) in WB
100 µg of total protein from Arabidopsis thaliana wild-type (1) and transgenic plants expressing GUS under HRT promoter (2) were extracted with write buffer containing 50 m M Tris-HCl p H 7.5,150 m M Na Cl, 10 m M Mg Cl 2,5 m M EDTA, 10% glycerol, 0.1% NP-40,0.1% protease inhibitor cocktail. The proteins were denatured by boiling for 5 min were separated on 10% SDS-PAGE and blotted 30 min to PVDF using tank... View More
Product Details
AS16-3689
Product Specifications
Species Reactivity
Tag
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: GUS
Target Information
GUS, also called beta glucuronidase, is a hydrolase commonly used in plant biology as a reporter enzyme to monitor gene expression, promoter activity, and tissue specific patterns. The E coli uidA gene encoding GUS is introduced into plants, and its activity is easily detected by colorimetric or fluorometric substrates that produce visible blue or fluorescent products upon cleavage. GUS is stable, has low background in most plant tissues, and allows precise spatial mapping via histochemical staining and quantitative assays. It supports studies of developmental regulation, hormone responses, and stress induced transcription, and can be combined with enhancers or nuclear localization signals for specific readouts. While widely reliable, potential endogenous glucuronidase activity or tissue permeability issues require controls, and alternative reporters such as luciferase and GFP are sometimes chosen depending on experimental needs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ECK1612; gurA; gusA; uidA
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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